FastQCFastQC Report
Mon 6 Jun 2016
ERR523185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10416439
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334280.32091581393602936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257120.24684059494804317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174780.1677924672721647No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112430.10793515903083578No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG193700.057.5645372
GTACCTG223500.050.4883961
ACCTGGG221150.049.8029563
CCTGGGG198100.038.285464
TATAACG10550.035.2152
CTGGGGG123250.028.5338945
GTATCAA441850.027.1778321
ATAACGC13350.026.7724113
TAACGCA14650.025.6765334
GTACATG422400.025.4226531
TACATGG430900.024.5669982
TGGGGGG177200.023.9752356
GGTATCA318400.023.9468731
CATGGGG265500.023.7137434
TCAACGC510550.023.2360884
ACATGGG446800.023.1034343
GTACCCG20450.023.0009381
TATCAAC516900.022.936092
CAACGCA517050.022.914715
ATCAACG518500.022.8472843
Produced by FastQC (version 0.11.5)