##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523185_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10416439
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06074158356805	33.0	31.0	34.0	27.0	34.0
2	31.391043810653525	33.0	31.0	34.0	27.0	35.0
3	31.340346542614036	33.0	31.0	34.0	26.0	36.0
4	33.623394712914845	35.0	33.0	37.0	30.0	37.0
5	33.70852697356553	35.0	34.0	37.0	30.0	37.0
6	33.938517280233675	35.0	35.0	37.0	29.0	37.0
7	33.81284122145774	35.0	34.0	37.0	30.0	37.0
8	33.893736813511794	35.0	34.0	37.0	29.0	37.0
9	35.03803439927983	37.0	34.0	39.0	28.0	39.0
10-11	34.98990336332791	37.0	34.5	39.0	29.5	39.0
12-13	35.008131905730934	37.0	35.0	39.0	29.0	39.0
14-15	35.73763298570654	37.0	34.5	40.0	29.0	41.0
16-17	35.89455547140439	37.5	35.5	40.0	28.5	41.0
18-19	35.74356893944274	38.0	34.0	40.0	27.5	41.0
20-21	35.835701817098915	38.0	35.0	40.0	28.0	41.0
22-23	35.68164485002984	38.0	35.0	40.0	28.0	41.0
24-25	35.65082539243977	37.5	34.5	40.0	27.0	41.0
26-27	35.53643783638535	37.0	35.0	40.0	27.0	41.0
28-29	35.287746080978344	37.0	34.5	40.0	27.0	41.0
30-31	35.10307586882619	36.5	34.5	40.0	26.0	41.0
32-33	35.0647204385299	37.5	34.0	40.0	25.5	41.0
34-35	34.90596901685883	37.5	34.0	40.0	25.0	41.0
36-37	34.72711086773512	37.0	33.5	40.0	25.0	41.0
38-39	34.55839726033052	36.5	33.5	40.0	24.0	41.0
40-41	34.50029885453176	37.0	33.0	40.0	24.0	41.0
42-43	34.29816811676236	36.0	33.0	40.0	23.5	41.0
44-45	34.2070061083255	37.0	33.0	40.0	23.5	41.0
46-47	34.00008222579713	36.5	33.0	40.0	22.0	41.0
48-49	33.85871721612348	37.0	33.0	39.0	21.0	41.0
50-51	32.8550851207404	35.5	31.5	38.0	19.5	40.0
52-53	33.08772422130058	36.0	31.5	38.5	21.0	39.5
54-55	33.937298245590455	37.0	33.0	39.0	22.0	40.5
56-57	34.07631187587236	36.5	33.0	40.0	22.5	41.0
58-59	34.00399632734373	37.0	33.0	40.0	21.5	41.0
60-61	33.75096302104779	36.5	33.0	39.0	22.0	41.0
62-63	33.56667897733573	36.0	32.0	39.0	21.5	41.0
64-65	33.17531183161539	36.0	32.0	39.0	20.0	41.0
66-67	32.98140784964996	36.0	32.0	38.0	20.5	40.0
68-69	32.638670854790206	35.0	31.5	37.5	19.0	40.0
70-71	32.430954090932616	35.0	31.0	37.0	18.5	39.5
72-73	32.10326849703627	35.0	31.0	37.0	18.5	39.0
74-75	31.73253642631613	35.0	31.0	36.5	18.0	39.0
76-77	31.6036556734984	35.0	30.0	37.0	18.0	38.5
78-79	31.335250559236222	35.0	30.0	36.0	18.0	38.0
80-81	31.070916365948094	35.0	29.5	36.0	18.0	37.5
82-83	30.76175437690366	34.0	29.0	35.5	17.0	37.0
84-85	30.52517616625029	34.0	29.0	35.0	17.5	37.0
86-87	30.272691031935196	34.0	29.0	35.0	16.5	37.0
88-89	30.116638997261923	34.0	29.0	35.0	17.0	36.5
90-91	29.897889240267233	34.0	29.0	35.0	17.0	36.0
92-93	29.64196118270361	34.0	27.0	35.0	15.0	36.0
94-95	29.539678147205585	34.0	27.0	35.0	15.0	36.0
96-97	29.346562534470756	34.0	27.0	35.0	12.5	36.0
98-99	29.252591216633633	34.0	26.0	35.0	11.0	36.0
100	28.004642373463714	32.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.054236712444446056
1101	2	0.03993936259270825
1101	3	0.07174350651685657
1101	4	0.13289205612672816
1101	5	0.07501182434657494
1101	6	0.16141621539610185
1101	7	0.08502299102227084
1101	8	0.062130065024632586
1101	9	0.13266724928320173
1101	10-11	0.05329129990794712
1101	12-13	0.12481676302421363
1101	14-15	0.04665086534381402
1101	16-17	0.1185722346387692
1101	18-19	0.10076167173145478
1101	20-21	0.04321470459025534
1101	22-23	-0.013498417108131378
1101	24-25	0.0034028764046567517
1101	26-27	0.043708401918173934
1101	28-29	-0.053841293921045974
1101	30-31	-0.09504440046173812
1101	32-33	-0.0921768752552552
1101	34-35	-0.14526926250496786
1101	36-37	-0.09596845430042578
1101	38-39	-0.18540153938703696
1101	40-41	-0.113871655586955
1101	42-43	-0.047034238366030934
1101	44-45	-0.11336610816233161
1101	46-47	-0.11689861901379572
1101	48-49	-0.19116783605860377
1101	50-51	-0.2696668817692114
1101	52-53	-0.13223064693727338
1101	54-55	-0.13922188208825048
1101	56-57	-0.08463449882269458
1101	58-59	-0.19504079874963054
1101	60-61	-0.13519852771999297
1101	62-63	-0.24128646937197118
1101	64-65	-0.20848535794348066
1101	66-67	-0.24339173607035036
1101	68-69	-0.2709164910046482
1101	70-71	-0.3453451960493936
1101	72-73	-0.26219015267399826
1101	74-75	-0.2575661773506184
1101	76-77	-0.2320721841808755
1101	78-79	-0.4048628671575685
1101	80-81	-0.39161407821049465
1101	82-83	-0.5854038952868521
1101	84-85	-0.45387002586255676
1101	86-87	-0.3711610817751634
1101	88-89	-0.4846326861479753
1101	90-91	-0.5477693080573118
1101	92-93	-0.4781310856613068
1101	94-95	-0.5801077339039118
1101	96-97	-0.5864633608334451
1101	98-99	-0.4663790244568524
1101	100	-0.6468008163766577
1102	1	0.07950022526825506
1102	2	0.0861563609441589
1102	3	0.08588481127735292
1102	4	0.09960575071961131
1102	5	0.07392876292474426
1102	6	0.07582746659705464
1102	7	0.13515260999088952
1102	8	0.09948873707406847
1102	9	0.09564978680979408
1102	10-11	0.10603654341419855
1102	12-13	-0.023322662088439472
1102	14-15	-0.012139984365404644
1102	16-17	0.0986537274501984
1102	18-19	0.06426521450300982
1102	20-21	0.04432315488151772
1102	22-23	0.016694903931288252
1102	24-25	-0.01955691582032415
1102	26-27	-0.01761906581331374
1102	28-29	0.07792623719597458
1102	30-31	-0.03929703959691011
1102	32-33	-0.10872453295298357
1102	34-35	-0.03275104728088252
1102	36-37	-0.4544920872858569
1102	38-39	-0.09929546169539805
1102	40-41	-0.7826344508208365
1102	42-43	-0.777195559112144
1102	44-45	-0.18113873247989432
1102	46-47	-0.14878896734667535
1102	48-49	-0.2567963706034462
1102	50-51	-0.2740070060699864
1102	52-53	-0.05676493974181085
1102	54-55	0.029183295217613647
1102	56-57	0.15633441135720716
1102	58-59	0.08838938025667176
1102	60-61	0.026362759027371396
1102	62-63	-0.25094930578701735
1102	64-65	0.019608730832523236
1102	66-67	0.01727490481844285
1102	68-69	0.03534048041627358
1102	70-71	-0.11367679622132698
1102	72-73	-0.06898549434487222
1102	74-75	-0.11767420300815346
1102	76-77	-0.2291875999077071
1102	78-79	-0.150872782690616
1102	80-81	-0.21850598974443258
1102	82-83	-0.30572394129434954
1102	84-85	-0.4180256453574849
1102	86-87	-0.30452016794014725
1102	88-89	-0.32599364875526504
1102	90-91	-0.2816032346991584
1102	92-93	-0.3892069786968868
1102	94-95	-0.49517118704459406
1102	96-97	-0.5969504514773725
1102	98-99	-0.5439427868819458
1102	100	-0.45336048082085156
1103	1	0.030142703751455002
1103	2	0.03154393492135554
1103	3	0.0384146742231124
1103	4	0.13551790177069734
1103	5	0.16666618260465782
1103	6	0.2529224858138619
1103	7	0.16065469340837524
1103	8	0.12287491134257067
1103	9	0.15090181720771056
1103	10-11	0.09644784292301978
1103	12-13	0.1617930942695125
1103	14-15	0.15200863201328474
1103	16-17	0.09172943346291618
1103	18-19	0.058737991765489994
1103	20-21	-0.013470906789983417
1103	22-23	0.01768637093903891
1103	24-25	-0.010870480691913542
1103	26-27	-0.06652400054660035
1103	28-29	-0.1924130816496259
1103	30-31	-0.15727236743476425
1103	32-33	-0.1901740184675873
1103	34-35	-0.2828167726975366
1103	36-37	-0.31851281889137795
1103	38-39	-0.1685951817496445
1103	40-41	-0.18380892610958455
1103	42-43	-0.12105851401697265
1103	44-45	-0.24307255274155892
1103	46-47	-0.17737588332359877
1103	48-49	-0.2630904767804978
1103	50-51	-0.3277283081608928
1103	52-53	-0.13227697523489468
1103	54-55	-0.052735825174544004
1103	56-57	0.14203709749100568
1103	58-59	0.07498272902100211
1103	60-61	0.060615197897625706
1103	62-63	0.052673578659842235
1103	64-65	-0.09405732019070712
1103	66-67	-0.019949518257007526
1103	68-69	-0.1566085030219213
1103	70-71	-0.11034836645468715
1103	72-73	-0.08636715941256057
1103	74-75	-0.026480177366291713
1103	76-77	-0.11163416225582168
1103	78-79	-0.10686699885395257
1103	80-81	-0.11078028691870401
1103	82-83	-0.008630296354482425
1103	84-85	-0.13249360663368037
1103	86-87	-0.8872269704465623
1103	88-89	-0.3190064673575641
1103	90-91	-0.5704205715116935
1103	92-93	-0.28756731507463584
1103	94-95	-0.7028957747244071
1103	96-97	-0.36533987952504887
1103	98-99	-0.25070727604704146
1103	100	-0.3417533882842356
1104	1	0.07890873569739298
1104	2	0.014089386865773434
1104	3	0.08869625142284221
1104	4	0.052308832998683386
1104	5	0.09557877888505573
1104	6	0.16343260492394052
1104	7	0.11596337494322029
1104	8	0.10946872260293361
1104	9	0.1402641544932166
1104	10-11	-0.04593004890913477
1104	12-13	0.08478469046671222
1104	14-15	0.018193405724041156
1104	16-17	0.07545715845277812
1104	18-19	0.1008419214519094
1104	20-21	-0.00625407232406161
1104	22-23	0.03315181470730977
1104	24-25	0.08282871339830677
1104	26-27	0.048807624257726445
1104	28-29	-0.027857329516521645
1104	30-31	0.03654064596454276
1104	32-33	0.05369494452074974
1104	34-35	0.019710053291852603
1104	36-37	0.050628482681510434
1104	38-39	0.009432278097968094
1104	40-41	0.0689411705663261
1104	42-43	-0.1087428228996643
1104	44-45	-0.0016254954835659419
1104	46-47	0.11117317049347974
1104	48-49	0.09149760938201013
1104	50-51	-0.13026724123602662
1104	52-53	-0.11546351153798895
1104	54-55	0.10877151618506531
1104	56-57	0.16294671857478704
1104	58-59	0.15510871717270902
1104	60-61	0.030393302865299177
1104	62-63	0.05027822252752401
1104	64-65	0.04022598323125948
1104	66-67	0.06622008964324522
1104	68-69	-0.05942800388439906
1104	70-71	0.002589441253647351
1104	72-73	0.11177682434396985
1104	74-75	0.04285181129553095
1104	76-77	0.037400159387686926
1104	78-79	-0.004457689769470363
1104	80-81	0.04858518980546478
1104	82-83	0.032367642743967195
1104	84-85	0.03674926351916241
1104	86-87	-0.06697716403779097
1104	88-89	-0.04885552277915295
1104	90-91	-0.020662838303667996
1104	92-93	-0.107961092224091
1104	94-95	-0.14150857493737945
1104	96-97	-0.17078948695157337
1104	98-99	-0.11062890479970022
1104	100	-0.0978099847961893
1105	1	0.12611496034830694
1105	2	0.09465517756289898
1105	3	0.16183088579891702
1105	4	0.08335228836511988
1105	5	0.12863266385033256
1105	6	0.24869295713811823
1105	7	0.06706692717397544
1105	8	0.028227410339667358
1105	9	0.1737110348593376
1105	10-11	0.1956499960531275
1105	12-13	0.2593204772698954
1105	14-15	0.18988701473681857
1105	16-17	0.1534365517398868
1105	18-19	0.14357333245036585
1105	20-21	0.11714632162001237
1105	22-23	0.08537530762948364
1105	24-25	0.06899269405348463
1105	26-27	0.1563460342242493
1105	28-29	-0.045991176473066275
1105	30-31	-0.014742020158927005
1105	32-33	0.008111292725573094
1105	34-35	-0.03437697219643354
1105	36-37	-0.08005867082461293
1105	38-39	-0.04003542268062432
1105	40-41	-0.045209482267992485
1105	42-43	0.016786132622385708
1105	44-45	-0.05160337115692215
1105	46-47	-0.09513052240791353
1105	48-49	-0.05923509082424516
1105	50-51	-0.16345320256402118
1105	52-53	0.020870450581632838
1105	54-55	0.1218792653323888
1105	56-57	0.17660968088040363
1105	58-59	0.14104550629132007
1105	60-61	0.10923392376145813
1105	62-63	0.11383491319992345
1105	64-65	0.1469594574650941
1105	66-67	0.22692827230850554
1105	68-69	0.21161860001716803
1105	70-71	0.14395052034167577
1105	72-73	0.13853515348906598
1105	74-75	0.11152337139945345
1105	76-77	0.24485786432921586
1105	78-79	0.16912069699221632
1105	80-81	0.21906222754900995
1105	82-83	0.14101019738704323
1105	84-85	0.1348572411676905
1105	86-87	0.10560010837334488
1105	88-89	-0.005717133896851578
1105	90-91	0.0027315273614654245
1105	92-93	0.03482790507814926
1105	94-95	0.06901483702544553
1105	96-97	0.0787961915035531
1105	98-99	0.08369400517674919
1105	100	0.14180836923582163
1106	1	0.09182742016010792
1106	2	0.03592238153619931
1106	3	0.13089966968299294
1106	4	0.14297255594649982
1106	5	0.09637517204057389
1106	6	0.13802978765427554
1106	7	0.15742844044622473
1106	8	-1.004971521895527
1106	9	0.15157674467653237
1106	10-11	0.13359487634271972
1106	12-13	0.15220181572766478
1106	14-15	0.109776665145084
1106	16-17	0.025208105976368245
1106	18-19	0.12521668642586548
1106	20-21	0.08771533775632889
1106	22-23	0.0987122717651161
1106	24-25	0.16667309337846348
1106	26-27	0.10809285769095567
1106	28-29	0.07869598087113161
1106	30-31	0.09573573833893789
1106	32-33	0.0445944354557426
1106	34-35	0.09311395172829862
1106	36-37	0.15901248396778556
1106	38-39	0.08849847758639129
1106	40-41	0.1430638730399707
1106	42-43	0.08539814445533978
1106	44-45	0.019026034284770788
1106	46-47	0.04538284810067239
1106	48-49	0.03554195074369915
1106	50-51	0.10962081592831652
1106	52-53	0.08522075470552437
1106	54-55	0.12188546244754406
1106	56-57	0.09987433975619098
1106	58-59	0.24330515499491412
1106	60-61	0.17275171152905244
1106	62-63	0.23638617804415674
1106	64-65	0.10945684276635603
1106	66-67	0.13137237619076103
1106	68-69	0.2204697088355374
1106	70-71	0.08689544901714896
1106	72-73	0.12481675970687434
1106	74-75	0.1977657199162799
1106	76-77	0.06316319340161058
1106	78-79	0.10803538858431594
1106	80-81	0.12110297665182301
1106	82-83	0.25383952846335944
1106	84-85	0.16386820764222776
1106	86-87	0.02972732937847411
1106	88-89	0.16905135825756545
1106	90-91	0.07071899839506912
1106	92-93	0.18694932833776434
1106	94-95	0.1747712684399474
1106	96-97	0.1684831776361584
1106	98-99	0.10847400758860104
1106	100	0.19677173739954767
1107	1	-0.09723767002641281
1107	2	-0.14945126401924824
1107	3	-0.14687124667353757
1107	4	0.03666224405373697
1107	5	-0.020554973201988957
1107	6	0.0053172757153987504
1107	7	0.0655334256804494
1107	8	0.08302648005860647
1107	9	0.02732381622194424
1107	10-11	0.0019461591540377299
1107	12-13	0.003924032460744797
1107	14-15	-0.030768845289763647
1107	16-17	0.05857643255806266
1107	18-19	0.061253831415932325
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2312	52-53	-9.672542065715106E-4
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2314	1	-0.465405654004325
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2314	90-91	0.134761040390444
2314	92-93	0.26424494145954824
2314	94-95	0.34823805389955353
2314	96-97	0.3876882879771877
2314	98-99	0.3900277130477221
2314	100	0.391224599221605
2315	1	-0.03756608244550108
2315	2	-0.09610013882205592
2315	3	-0.09287575503766021
2315	4	-0.0715092171005196
2315	5	-0.07746523894364543
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2315	7	-0.08411749344642061
2315	8	0.005134304076797491
2315	9	-0.1331132924056746
2315	10-11	-1.4554837354767685
2315	12-13	-0.11179456228911988
2315	14-15	-0.18344320402000847
2315	16-17	-0.10871179768380301
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2315	20-21	-0.05389204049452445
2315	22-23	-0.04039760121907676
2315	24-25	-0.08701972794934676
2315	26-27	-0.11653724419272038
2315	28-29	-0.039236685968013774
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2315	100	0.5190244508070485
2316	1	-0.12214311010362167
2316	2	-0.37488513972304105
2316	3	-0.26535249972231156
2316	4	-0.23696178581134575
2316	5	-0.2759653798419208
2316	6	-0.16626972072101154
2316	7	-0.1438796064782366
2316	8	-0.07833721400899663
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2316	10-11	-0.3520249738046246
2316	12-13	-0.2655528696633098
2316	14-15	-0.23345289541097003
2316	16-17	-0.3858887384081129
2316	18-19	-0.24857039648914991
2316	20-21	-0.13362795174732156
2316	22-23	-0.19213428017896916
2316	24-25	-0.14187094592985972
2316	26-27	-0.16523139197143166
2316	28-29	-0.03857736216679086
2316	30-31	-0.1666817954976878
2316	32-33	-0.15817157533908954
2316	34-35	-0.16508898042451392
2316	36-37	-0.04294301771849973
2316	38-39	-0.13492118530435704
2316	40-41	-0.12475271378716712
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2316	46-47	-1.624459288221729
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2316	54-55	-0.04353409859238866
2316	56-57	-0.17666227087222808
2316	58-59	-0.27447081839935095
2316	60-61	-0.16760570698268396
2316	62-63	-0.15166250088994815
2316	64-65	-0.3388190277619785
2316	66-67	-0.3031097268288008
2316	68-69	-0.08278771942139684
2316	70-71	-0.06554514675255518
2316	72-73	-0.13596850072158162
2316	74-75	-0.04742855457943307
2316	76-77	-0.17747850222147576
2316	78-79	-0.28593127490163184
2316	80-81	-0.04149920830180065
2316	82-83	-0.07295185299566498
2316	84-85	0.033387720531429466
2316	86-87	0.04379200819482776
2316	88-89	0.14458245439220718
2316	90-91	0.2572405242758329
2316	92-93	0.3463101959036301
2316	94-95	0.3399226759267613
2316	96-97	0.4183189516667767
2316	98-99	0.37806218940955105
2316	100	0.16315918565781473
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	18735.0
1	1716.0
2	1088.0
3	777.0
4	686.0
5	674.0
6	788.0
7	2408.0
8	4696.0
9	6276.0
10	10400.0
11	19011.0
12	29859.0
13	38629.0
14	44482.0
15	50267.0
16	57049.0
17	66269.0
18	78658.0
19	90795.0
20	103986.0
21	117813.0
22	132068.0
23	143497.0
24	150052.0
25	159101.0
26	173063.0
27	192991.0
28	220427.0
29	258464.0
30	308959.0
31	378519.0
32	474708.0
33	624615.0
34	884972.0
35	1370457.0
36	1381046.0
37	1378912.0
38	1199716.0
39	239590.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.97232224801624	17.139868203744836	12.015241309517377	23.87256823872155
2	16.57804435137938	19.729538937873368	39.82868231099996	23.863734399747294
3	19.47386637042082	25.8857137134145	29.03861917488238	25.601800741282304
4	13.040729136723728	15.68089819906619	36.31864727014296	34.95972539406712
5	13.578723098550805	36.27152881528752	33.56505605778678	16.584692028374903
6	31.30544910708734	34.488803195385486	19.31827785061854	14.887469846908633
7	28.734720113099964	31.456457562053913	21.618808721548987	18.190013603297132
8	27.229625251996847	33.66725973252842	20.362451952761994	18.740663062712745
9	28.068621917848567	15.68182629838798	19.80081438904794	36.44873739471551
10-11	25.69091072872503	25.07825208083553	28.157530648811864	21.073306541627584
12-13	26.017669073481226	23.08359534273033	27.973532146222585	22.925203437565855
14-15	23.55356399612429	24.43820923424484	26.048657893697367	25.9595688759335
16-17	23.204660574915675	26.831587475010448	26.702021205891093	23.261730744182785
18-19	22.88611855266026	26.24558539821284	28.33897885851475	22.529317190612147
20-21	23.72172911917628	25.664507692293625	28.17673057417528	22.437032614354813
22-23	23.435910894206792	25.40218691752837	28.060624975459685	23.101277212805158
24-25	23.024603316399382	25.506997193789253	28.094236020324594	23.37416346948677
26-27	22.970802975201934	25.501090212550302	28.216016351696595	23.312090460551165
28-29	22.961710078875715	25.983071050317985	28.120222097240376	22.934996773565928
30-31	23.66994516998277	25.608081120691235	28.136628667746493	22.585345041579497
32-33	23.300353084139818	25.721899512981423	28.094096355416347	22.883651047462415
34-35	23.471614139462822	25.891008207162592	27.957192363718157	22.680185289656425
36-37	23.45974424870923	25.87137601423771	27.838050326949006	22.830829410104048
38-39	23.4384336597697	26.169554947951077	27.578336114774306	22.813675277504917
40-41	23.44976183056582	25.87047288574244	27.775207518429706	22.904557765262034
42-43	23.248238364079317	25.9607842684358	27.512009382302853	23.278967985182028
44-45	23.102711955594245	26.083078776412012	27.632932648253693	23.18127661974005
46-47	23.07760778977305	25.830519490454908	27.474001323546236	23.617871396225805
48-49	23.142522850124976	25.931184847760868	27.533687829362634	23.392604472751515
50-51	22.97207931488134	26.34058772599988	27.506660945347576	23.180672013771197
52-53	23.03228412901717	26.434313463875707	27.115362265555614	23.418040141551508
54-55	22.824052585588362	26.192682080237145	27.456695416068026	23.526569918106464
56-57	23.08422830524793	26.26284224840142	27.09437144059617	23.55855800575448
58-59	23.086926851429418	26.23067046279239	27.347134714337688	23.33526797144051
60-61	23.454556328878915	26.318971241862837	27.338266784257026	22.888205645001225
62-63	23.2164215514164	26.28695072149405	27.3681630272905	23.128464699799054
64-65	23.10470318307818	26.076857276868864	27.346622476257505	23.471817063795452
66-67	23.074177494680047	26.091989992604475	27.195384184591802	23.638448328123676
68-69	22.761475886811905	26.1149843644637	27.367199017731185	23.75634073099321
70-71	22.86558622684018	25.930161080047636	27.464388586304395	23.739864106807786
72-73	22.769470668060837	26.326724118589752	27.06215424779255	23.84165096555687
74-75	22.856254545522813	26.36518996813555	27.004953124645752	23.773602361695886
76-77	22.72170710068058	26.026490754767405	27.174491491028167	24.07731065352385
78-79	22.6328421566184	26.088373302389673	27.287006983406	23.991777557585927
80-81	22.760268066921633	26.22357557443943	26.961860736951103	24.05429562168783
82-83	22.90571707252272	26.170532942217523	27.08775390219361	23.835996083066142
84-85	22.88085894205252	26.37399829509738	26.73197104467092	24.013171718179184
86-87	22.687913800818034	26.28834672975577	27.001184460920108	24.022555008506092
88-89	22.57572288771528	26.007278169765296	27.15293668440944	24.264062258109984
90-91	23.101976992178557	25.895283972290805	27.09030518893781	23.912433846592833
92-93	22.892689136089434	26.019148818418287	27.086492118377137	24.00166992711514
94-95	22.690391768095523	25.844985078510685	27.128638934615434	24.33598421877836
96-97	22.635987160662356	25.74558674844039	27.26138333586439	24.35704275503286
98-99	22.70445620345513	25.77253672995687	26.962870658256637	24.560136408331363
100	22.713865414385044	25.720084557314614	27.107874695972324	24.458175332328018
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8357.0
1	9408.0
2	10861.5
3	8760.0
4	4734.0
5	3458.5
6	4471.5
7	7352.5
8	11046.5
9	12641.5
10	11452.5
11	10103.5
12	10171.5
13	10913.0
14	11815.5
15	12401.5
16	13102.5
17	13966.5
18	14926.0
19	15915.0
20	17004.0
21	18694.5
22	21039.5
23	24532.5
24	28449.5
25	33290.0
26	40616.0
27	49578.0
28	58901.0
29	70585.0
30	85687.0
31	100761.0
32	116747.0
33	134518.5
34	153225.5
35	171535.0
36	192636.5
37	215111.0
38	233567.5
39	250743.0
40	264965.0
41	277782.0
42	292860.5
43	309944.0
44	327072.5
45	345086.0
46	365170.0
47	388260.0
48	416772.5
49	437439.5
50	451933.0
51	449185.5
52	427081.0
53	413115.5
54	400977.5
55	380693.5
56	351612.5
57	320447.0
58	290926.0
59	255864.5
60	215167.5
61	176703.0
62	145211.0
63	115670.0
64	87661.0
65	66353.5
66	50123.5
67	38864.0
68	30656.0
69	23698.5
70	18600.0
71	14592.0
72	11751.5
73	9280.5
74	7209.5
75	5501.0
76	4012.5
77	2756.0
78	1875.5
79	1265.0
80	857.5
81	633.5
82	456.0
83	330.0
84	263.5
85	188.0
86	134.0
87	123.0
88	105.0
89	72.0
90	54.0
91	43.5
92	34.0
93	25.0
94	15.0
95	15.5
96	15.0
97	9.0
98	11.5
99	9.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14623999622135742
2	0.07174236800119503
3	0.30980837117176036
4	0.3082051361314553
5	0.3104035841807358
6	0.2587928561766646
7	0.25964727485083916
8	0.2584760492525324
9	0.2691706829944475
10-11	0.24635098424711172
12-13	0.2563592029867405
14-15	0.2306402408731045
16-17	0.23852201313711913
18-19	0.25156389818055863
20-21	0.2534215387811516
22-23	0.24360532423796655
24-25	0.24365812539198858
26-27	0.24332691815312316
28-29	0.25426635724550395
30-31	0.23961643705684832
32-33	0.252303114336867
34-35	0.2361795619405058
36-37	0.24428213903043067
38-39	0.2452085592782716
40-41	0.26210012846040764
42-43	0.24781021614008394
44-45	0.23388511179300336
46-47	0.23674117421510366
48-49	0.2460677780573572
50-51	0.2390068237331395
52-53	0.2551879773884338
54-55	0.26482178794499733
56-57	0.2606120959379688
58-59	0.2582360440069778
60-61	0.23998124503009136
62-63	0.26710183777776647
64-65	0.26070329793127955
66-67	0.2661370166906368
68-69	0.2711483262178178
70-71	0.2905743507930109
72-73	0.30040976575584033
74-75	0.2799181178903846
76-77	0.276630046026286
78-79	0.2738747858073186
80-81	0.26009848471248187
82-83	0.27083631939859676
84-85	0.2816269552387337
86-87	0.2744795990261163
88-89	0.28308138702679486
90-91	0.27687965148166277
92-93	0.25694001568098274
94-95	0.2774796645955494
96-97	0.276788449488352
98-99	0.26244573601400634
100	0.28453101870994496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0416439E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.42432572766545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90753894245184	40.40851186348904
2	15.608916336940185	17.302273266289028
3	5.336134171827243	8.872549153976381
4	2.2294007317333366	4.942521293323365
5	1.1856296161729392	3.2856361019567966
6	0.6940456745639167	2.3080208122144685
7	0.4164702020210369	1.6157806092876408
8	0.28673987811980384	1.2713891523218477
9	0.20703045164554504	1.032707086879354
>10	1.0263879522870984	9.991025836977423
>50	0.06635980158320241	2.5063562535393062
>100	0.03222127551812615	3.241409636649226
>500	0.0020996936972880473	0.7769919244392558
>1k	8.055704192476737E-4	0.842308530015331
>5k	1.2205612175073569E-4	0.41378054297639905
>10k+	9.764489740058855E-5	1.1887379356652192
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33428	0.32091581393602936	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25712	0.24684059494804317	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17478	0.1677924672721647	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11243	0.10793515903083578	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9200419644371748E-5
2	0.0	0.0	0.0	0.0	1.9200419644371748E-5
3	0.0	0.0	0.0	0.0	1.9200419644371748E-5
4	0.0	0.0	0.0	0.0	1.9200419644371748E-5
5	0.0	0.0	0.0	9.600209822185874E-6	1.9200419644371748E-5
6	0.0	0.0	0.0	9.600209822185874E-6	3.8400839288743496E-5
7	0.0	0.0	0.0	9.600209822185874E-6	6.720146875530112E-5
8	0.0	0.0	0.0	9.600209822185874E-6	6.720146875530112E-5
9	0.0	0.0	0.0	2.8800629466557622E-5	6.720146875530112E-5
10-11	0.0	0.0	0.0	2.8800629466557622E-5	6.720146875530112E-5
12-13	0.0	0.0	0.0	2.8800629466557622E-5	6.720146875530112E-5
14-15	4.800104911092937E-6	0.0	0.0	2.8800629466557622E-5	1.2000262277732343E-4
16-17	9.600209822185874E-6	0.0	0.0	4.320094419983643E-5	1.7280377679934573E-4
18-19	9.600209822185874E-6	0.0	0.0	7.200157366639406E-5	2.1120461608808923E-4
20-21	9.600209822185874E-6	0.0	0.0	1.5360335715497398E-4	2.2560493082136803E-4
22-23	1.9200419644371748E-5	0.0	0.0	3.9840870762071376E-4	2.496054553768327E-4
24-25	1.9200419644371748E-5	0.0	0.0	9.264202478409369E-4	3.0720671430994797E-4
26-27	1.9200419644371748E-5	0.0	0.0	0.001968043013548104	3.0720671430994797E-4
28-29	1.9200419644371748E-5	0.0	0.0	0.004795304806181844	3.0720671430994797E-4
30-31	1.9200419644371748E-5	0.0	0.0	0.014635519873922365	3.0720671430994797E-4
32-33	1.9200419644371748E-5	0.0	0.0	0.038516041806609724	3.8400839288743496E-4
34-35	1.9200419644371748E-5	0.0	0.0	0.07673447710873169	4.3680954690945725E-4
36-37	1.9200419644371748E-5	0.0	0.0	0.13460454191686813	4.5120986164273605E-4
38-39	1.9200419644371748E-5	0.0	0.0	0.22331528077877671	4.704102812871078E-4
40-41	2.8800629466557622E-5	0.0	0.0	0.3291767944880203	5.040110156647584E-4
42-43	2.8800629466557622E-5	0.0	0.0	0.4295181875495071	5.664123795089666E-4
44-45	2.8800629466557622E-5	0.0	0.0	0.5518872620479993	5.952130089755241E-4
46-47	2.8800629466557622E-5	0.0	0.0	0.6932791523091528	6.384139531753607E-4
48-49	2.8800629466557622E-5	4.800104911092937E-6	0.0	0.8438872439996048	7.104155268417547E-4
50-51	2.8800629466557622E-5	1.9200419644371748E-5	0.0	1.002511510891582	7.248158415750335E-4
52-53	2.8800629466557622E-5	2.8800629466557622E-5	0.0	1.1675055170005795	7.776169955970558E-4
54-55	2.8800629466557622E-5	2.8800629466557622E-5	0.0	1.3616025591855334	8.304181496190781E-4
56-57	2.8800629466557622E-5	2.8800629466557622E-5	0.0	1.5790473116580437	8.35218254530171E-4
58-59	2.8800629466557622E-5	2.8800629466557622E-5	0.0	1.8206941930922844	8.40018359441264E-4
60-61	2.8800629466557622E-5	3.360073437765056E-5	0.0	2.062518678408235	8.448184643523569E-4
62-63	2.8800629466557622E-5	3.8400839288743496E-5	0.0	2.3180378630355345	8.640188839967286E-4
64-65	2.8800629466557622E-5	3.8400839288743496E-5	0.0	2.575587492040226	8.640188839967286E-4
66-67	2.8800629466557622E-5	3.8400839288743496E-5	0.0	2.8700115269719335	8.784191987300075E-4
68-69	2.8800629466557622E-5	3.8400839288743496E-5	0.0	3.2106509719876435	8.880194085521933E-4
70-71	2.8800629466557622E-5	3.8400839288743496E-5	0.0	3.578598213842562	9.072198281965651E-4
72-73	2.8800629466557622E-5	3.8400839288743496E-5	0.0	3.9152151709427763	0.0010464228706182602
74-75	2.8800629466557622E-5	3.8400839288743496E-5	4.800104911092937E-6	4.256891438619283	0.0010464228706182602
76-77	2.8800629466557622E-5	3.8400839288743496E-5	9.600209822185874E-6	4.633709274349901	0.001070423395173725
78-79	2.8800629466557622E-5	3.8400839288743496E-5	9.600209822185874E-6	5.061518624551058	0.0010848237099070038
80-81	2.8800629466557622E-5	3.8400839288743496E-5	9.600209822185874E-6	5.496849739147899	0.0011184244442846542
82-83	2.8800629466557622E-5	3.8400839288743496E-5	9.600209822185874E-6	5.942477078779033	0.0011760257032177694
84-85	3.8400839288743496E-5	3.8400839288743496E-5	9.600209822185874E-6	6.396519002319314	0.0011808258081288624
86-87	3.8400839288743496E-5	3.8400839288743496E-5	9.600209822185874E-6	6.866550075318447	0.0012000262277732342
88	3.8400839288743496E-5	3.8400839288743496E-5	9.600209822185874E-6	7.237550183896819	0.00120962643759542
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	19370	0.0	57.564537	2
GTACCTG	22350	0.0	50.488396	1
ACCTGGG	22115	0.0	49.802956	3
CCTGGGG	19810	0.0	38.28546	4
TATAACG	1055	0.0	35.215	2
CTGGGGG	12325	0.0	28.533894	5
GTATCAA	44185	0.0	27.177832	1
ATAACGC	1335	0.0	26.772411	3
TAACGCA	1465	0.0	25.676533	4
GTACATG	42240	0.0	25.422653	1
TACATGG	43090	0.0	24.566998	2
TGGGGGG	17720	0.0	23.975235	6
GGTATCA	31840	0.0	23.946873	1
CATGGGG	26550	0.0	23.713743	4
TCAACGC	51055	0.0	23.236088	4
ACATGGG	44680	0.0	23.103434	3
GTACCCG	2045	0.0	23.000938	1
TATCAAC	51690	0.0	22.93609	2
CAACGCA	51705	0.0	22.91471	5
ATCAACG	51850	0.0	22.847284	3
>>END_MODULE