FastQCFastQC Report
Mon 6 Jun 2016
ERR523185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10416439
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305900.2936704184606659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260820.25039267258225195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171640.16477800138799834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG197400.057.4987372
GTACCTG225000.050.8393671
ACCTGGG226850.049.365973
CCTGGGG200450.038.1672444
TATAACG10800.030.0271192
CTGGGGG120700.029.1230455
GTATCAA441400.025.6060541
ATAACGC12850.025.2341373
TGGGGGG159900.024.5697216
GGTATCA324750.024.524761
GTACCCG19150.024.3056341
TAACGCA14000.023.4970044
GTACATG414900.022.9694631
CATGGGG258050.022.1994534
TACATGG424550.021.996692
TCAACGC508950.021.8280434
CAACGCA516950.021.5447885
ATGGGGG158550.021.3703425
TATCAAC525250.021.2602672
ATCAACG522450.021.2460213
Produced by FastQC (version 0.11.5)