FastQCFastQC Report
Mon 6 Jun 2016
ERR523184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8133495
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284380.3496405911603806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236160.29035488433938916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131150.1612467948895278No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127900.15725097267533822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG115050.043.5374532
ACCTGGG123300.038.9455073
GTACCTG137650.037.0040781
TATAACG8800.036.8779222
TAACGCA10600.031.5018794
ATAACGC11450.029.5747933
CCTGGGG121250.026.8415954
GTATCAA425650.024.1432741
GTACATG297550.023.267261
GGTATCA316550.022.554171
TACATGG303850.022.305132
GTATAAC22800.022.2785171
ACATGGG301700.021.5128773
TCAACGC482200.021.0868874
CATGGGG189650.020.955014
CAACGCA485350.020.9300065
ATCAACG489150.020.7300993
TATCAAC496200.020.5874772
GAGTACT260250.020.53385412-13
AACGCAG500400.020.423426
Produced by FastQC (version 0.11.5)