FastQCFastQC Report
Mon 6 Jun 2016
ERR523184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8133495
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262530.3227763710434444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224850.2764494230340093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129070.1586894686724465No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128120.15752145910214488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG113750.045.1238862
ACCTGGG126500.039.1245273
GTACCTG138150.037.587141
TATAACG7400.035.5706332
CCTGGGG118050.027.910054
ATAACGC11050.026.3718533
TAACGCA10200.026.2653524
GTACATG294250.022.7622181
TATCACG7850.022.7535512
GGTATCA315250.022.7379321
GTATCAA419450.022.5727581
TACATGG301350.021.8057612
ACATGGG302250.020.8065953
CATGGGG181500.020.2506374
TCAACGC469500.019.8315854
CAACGCA475800.019.5791175
ATCAACG480200.019.3506583
TATCAAC487100.019.183752
CTGGGGG75200.019.1257085
AACGCAG487900.019.1018946
Produced by FastQC (version 0.11.5)