FastQCFastQC Report
Mon 6 Jun 2016
ERR523183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5721193
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT436660.7632324237270094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218660.38219301463873007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174800.30553068214968454No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT115930.20263256282387257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110180.1925822114373698No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84010.14684000347479975No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA70690.12355814600206637No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG66720.11661903382738531No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA62010.10838648512644128No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC58300.10190182362314992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50800.034.796822
TATCACG5150.030.124662
GTACATG208250.029.0113451
GTACCTG61350.028.9685651
ACCTGGG60800.028.147013
TACATGG215600.027.627612
ACATGGG217050.026.9677093
ATGGGGG102850.026.0080725
CATGGGG155950.025.5321144
ATAACGC5900.023.9058253
TATAACG5750.022.0755422
TGGGGGG106550.021.2964946
GAGTACT179600.019.8551312-13
AGAGTAC270400.019.50181610-11
TAACGCA6350.019.2481234
CCTGGGG59850.018.3797864
AGTACTT188700.018.06353812-13
GTACTTT203450.017.4469614-15
GAGTACA158950.016.1503471
GTATAAC15300.015.6721471
Produced by FastQC (version 0.11.5)