FastQCFastQC Report
Mon 6 Jun 2016
ERR523183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5721193
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT453130.7920201258723487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204920.35817704454298255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174090.3042896822393511No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT119540.20894243560739867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105870.18504881761548683No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC97860.17104824116228903No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC87980.1537791156494808No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA72540.12679173731772378No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG71080.12423982200915089No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC58190.10170955603140813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55150.034.772062
GTACCTG65400.029.8452171
GTACATG198500.029.0018231
ACCTGGG66550.027.9641843
TACATGG206450.027.2973252
ACATGGG211500.025.8863373
CATGGGG145000.025.1506254
TATAACG5050.025.1297512
ATGGGGG97950.024.1331925
ATAACGC4950.023.7350353
TAACGCA5350.023.7172874
TATCACG5400.023.500972
TGGGGGG98150.021.1632926
GAGTACT188500.019.83920912-13
AGAGTAC275250.019.56416910-11
CCTGGGG65350.018.6255554
AGTACTT198500.018.40161912-13
GAGTACA153650.017.7847581
GTACTTT212050.017.61087414-15
AGTACAT150250.016.454592
Produced by FastQC (version 0.11.5)