FastQCFastQC Report
Mon 6 Jun 2016
ERR523182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5603094
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184040.32846138222917554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147790.26376498413198135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83660.14931036316720728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82000.047.9988172
ACCTGGG88800.043.157843
GTACCTG110450.036.3589321
GGTATCA133950.035.3162231
TATAACG7000.034.9320682
GTATCAA203550.031.9499381
TAACGCA8000.028.8019034
CCTGGGG85550.028.2525844
TATCACG4750.027.7193742
TCAACGC238550.027.0451744
ATAACGC8700.027.0250113
CAACGCA239900.026.871465
ATCAACG242100.026.6291793
TATCAAC246400.026.2601182
AACGCAG250250.026.0347236
GTACATG193000.023.3414151
ACGCAGA280050.023.0615147
CGCAGAG285750.022.600088
TACATGG195350.022.1218322
ACATGGG190200.021.7316883
Produced by FastQC (version 0.11.5)