FastQCFastQC Report
Mon 6 Jun 2016
ERR523182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5603094
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT179300.32000177045039757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171730.3064913777994801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147430.26312248197156785No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT93520.16690778344964408RNA PCR Primer, Index 23 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82110.1465440344209824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82250.048.8044432
ACCTGGG91300.043.1925773
TAACGCA7300.037.987724
TATAACG7500.036.9766962
GTACCTG111050.036.8903051
ATAACGC8000.036.426693
GGTATCA132100.035.925351
CCTGGGG77050.032.025864
GTATCAA199800.030.6733761
TCAACGC231550.026.0229994
CAACGCA233950.025.7569585
ATCAACG235400.025.6575183
TATCAAC242000.025.0559982
AACGCAG242050.025.0289466
TATCACG5100.023.9629612
GTACATG190000.022.7001321
ACGCAGA271700.022.2627987
TACATGG192500.021.6585942
CGCAGAG280250.021.58348
ACATGGG189100.021.4256363
Produced by FastQC (version 0.11.5)