FastQCFastQC Report
Mon 6 Jun 2016
ERR523181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5489417
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1567232.8550026350703543No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT428520.7806293455206628No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC373370.680163303316181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355140.6469539479329043No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA333980.6084070494189091No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG312010.5683845843738962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272730.4968287160549108No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA249620.45472952774402087No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC237770.4331425359013535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171880.3131115745078211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG255050.027.8207381
CATTCCG9450.026.3496829
TACATGG268350.025.9841462
ACATGGG258500.025.9566293
CATGGGG112550.023.9790174
GAGTACT245500.023.05041112-13
CATGGGA114200.022.5209244
CATGGGT34050.022.2317564
GTACTTT266550.021.11647214-15
TAGACGC2701.1405064E-920.8969294
AGTACTT263400.020.52079812-13
CGTATCA5050.020.48087994
ATGGGGG59100.020.4462935
AGAGTAC383700.020.07423810-11
ATGGGAT50650.019.4015225
TTAGACG2251.8403334E-618.8080963
CCGTATC6150.018.34650894
GAGTACG5700.018.1489931
AGTACAT187550.018.1255072
GAGTACA200550.018.0539721
Produced by FastQC (version 0.11.5)