FastQCFastQC Report
Mon 6 Jun 2016
ERR523181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5489417
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1559702.8412853313931152No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT417120.7598621128618941No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC385240.701786728900355No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA329670.6005555781242343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309160.5631927762092039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255290.46505849346114536No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA254850.4642569511479999No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC236300.4304646559006175No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG158280.2883366302833252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156530.28514867790149667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG9100.027.896399
GTACATG253050.027.3601721
TACATGG264550.025.7807752
ACATGGG257200.025.5803433
CATGGGG110700.023.9431214
GAGTACT245900.023.01821112-13
CATGGGA115200.021.7840214
ATGGGCG8300.021.5152745
GTACTTT265400.021.18178714-15
ATGGGGG55450.021.1027555
TTAGGCA14750.021.0281584
CATGGGT34350.020.7953434
GCGTATC1405.18818E-420.157681
AGTACTT264550.020.08937312-13
ATGGGTA19900.019.8366155
AGAGTAC386300.019.62359810-11
ATGGGAT49300.018.9691355
AGTACAT188050.018.096792
GAGTACA202650.018.0804061
ACTTTTT317350.017.94064916-17
Produced by FastQC (version 0.11.5)