FastQCFastQC Report
Mon 6 Jun 2016
ERR523180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10472619
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392770.37504467602612107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335260.3201300457889283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184590.1762596347675782No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118740.11338138053146019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG99500.026.1800042
GTACCTG115500.023.2903581
GTATCAA588600.023.0908371
GGTATCA444700.022.9379481
GAGTACT313950.021.6355112-13
GTACATG538450.021.1015171
CATGGGG296050.020.5817074
TCAACGC664400.020.295094
ACCTGGG126500.020.1835983
GTACTTT335750.020.1611414-15
CAACGCA671100.020.090935
TACATGG558050.020.0617732
ATCAACG678700.019.8627383
TATCAAC683700.019.7653372
AACGCAG688350.019.633916
TATAACG8150.019.6157232
ACATGGG555150.019.6078473
ATGGGGG152000.018.1861975
AGTACTT337000.018.14779312-13
ACGCAGA756800.017.8519637
Produced by FastQC (version 0.11.5)