FastQCFastQC Report
Mon 6 Jun 2016
ERR523180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10472619
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341930.32649903524610224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327830.31303535438461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188070.17958258578871245No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT123930.11833716093366904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG96850.026.737782
GTACCTG117650.022.57941
GGTATCA464150.022.4677221
GTATCAA600100.021.8671441
GAGTACT329800.020.47996512-13
GTACATG540700.020.1216141
CATGGGG301800.019.6195664
TACATGG557450.019.298042
GTACTTT352700.019.01562114-15
ACCTGGG134150.018.9165153
TCAACGC688450.018.7851264
ACATGGG556550.018.6943993
CAACGCA694350.018.6119695
ATCAACG704750.018.3639853
AACGCAG706850.018.309346
TATCAAC710250.018.2960452
AGTACTT354900.018.09121712-13
ATGGGGG150050.017.444455
ACGCAGA782250.016.5385197
ACTTTTT421500.016.22858616-17
Produced by FastQC (version 0.11.5)