FastQCFastQC Report
Mon 6 Jun 2016
ERR523179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7800404
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT457660.5867132010085632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245640.31490676636748555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204400.2620377098416954No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT134490.17241414675445016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110520.14168496913749595No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC106040.13594167686699304No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC94500.12114757133092081No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80010.10257161039351295No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG267900.027.0468351
TACATGG275350.025.7147142
ACATGGG283700.024.1806813
CATGGGG190500.022.1130814
GAGTACT215000.020.69420412-13
TATAACG5150.019.1714022
ATGGGGG110500.019.10375
AGAGTAC335250.018.98655710-11
GTACTTT232950.018.81791514-15
GAGTACA213700.017.4263821
AGTACTT229800.016.86712612-13
GTATAGG20800.016.7284221
AGTACAT210700.016.2222732
ACTTTTT287200.015.6225516-17
TATACTG36350.015.2619855
CATGGGA99700.015.0900644
TACCTGG52200.014.7711792
TACTTTT265800.014.4594214-15
GTATATA38200.014.4015451
GTGTAGC41350.014.3278691
Produced by FastQC (version 0.11.5)