FastQCFastQC Report
Mon 6 Jun 2016
ERR523179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7800404
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT462670.5931359452664247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226120.28988242147458004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195990.25125621698568434No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT134480.1724013269056321No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC111430.14285157537994184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108050.1385184664794285No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC95970.12303208910717958No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA81980.10509712061067607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG268900.025.6573981
TACATGG274300.024.7090782
ACATGGG281800.023.513953
CATGGGG191450.021.5764984
GAGTACT218500.020.21461512-13
AGAGTAC349100.018.94804210-11
GTACTTT233150.018.59823414-15
ATGGGGG105300.018.0747095
AGTACTT231400.017.35060512-13
CATGGGA101100.016.5945134
TACCTGG55750.016.2715722
GAGTACA218450.015.3070261
ACTTTTT288450.015.21730416-17
TACTTTT268800.014.86381914-15
AGTACAT210250.014.6874512
ATTATAC30150.014.6517013
GTATAGC22300.014.3409311
GTATACG5007.6761353E-1014.1089461
GTATTAG24050.014.0796141
GTACCTG69950.013.9845641
Produced by FastQC (version 0.11.5)