FastQCFastQC Report
Mon 6 Jun 2016
ERR523178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7682317
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304610.3965079806001236No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT282070.36716787396302447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254880.3317749059300729No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161330.21000174817050637No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115190.1499417428361782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG111100.050.830382
GTACCTG134000.042.890961
ACCTGGG131050.042.5177273
CCTGGGG121400.031.2544844
TATAACG8050.029.7898752
ATAACGC9150.025.694353
GTACATG353250.024.3517931
CATGGGG215500.024.3268074
ATGGGGG138200.023.9833725
TACATGG358650.023.4811382
CTGGGGG81500.022.9014025
TGGGGGG145700.022.5437186
ACATGGG363800.022.450493
TAACGCA9950.022.209174
GGGTTAG29400.021.7565961
GTACACG15850.021.0682491
GAGTACT269350.020.741512-13
TATCACG7800.020.4964522
GTATAAC19600.019.4369581
AGAGCGC25350.018.721848
Produced by FastQC (version 0.11.5)