FastQCFastQC Report
Mon 6 Jun 2016
ERR523178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7682317
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT287530.37427510476331555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267230.3478507851212076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237590.3092686750624844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152750.19883324262719176No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT148450.19323597294930683No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99040.12891943927854058No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG120800.052.8347362
ACCTGGG138000.045.6311653
GTACCTG142350.045.5178831
CCTGGGG125700.034.843084
CTGGGGG77100.027.5498925
TATAACG8400.027.4159642
TATCACG8150.024.7969112
TAACGCA9950.024.0870114
TGGGGGG130450.024.0638686
GTACATG353800.023.9888671
CATGGGG211800.023.9847724
ATAACGC9450.023.8696083
TACATGG359850.023.2218592
ATGGGGG130750.022.5711565
ACATGGG365250.022.1939563
GGGTTAG26650.021.1726071
AGAGCGC26900.020.0943858
GTACACG16900.020.0325451
GTGGTTA48550.019.6606161
GAGTACT274650.019.60482612-13
Produced by FastQC (version 0.11.5)