FastQCFastQC Report
Mon 6 Jun 2016
ERR523177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909247
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183130.46845338757054744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138330.3538533124154089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87990.22508171010938938No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45280.11582793310322935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG36100.040.7562752
GGTATCA97800.040.474641
TATCACG4350.037.8212742
GTATCAA167550.035.4800951
ACCTGGG41300.034.0264973
GTACCTG51550.029.1264741
TCAACGC202400.028.8842684
ATCAACG203700.028.6105843
CAACGCA205600.028.4100325
AACGCAG212050.027.6949626
TATCAAC212000.027.6051942
GTACATG174900.027.5034371
ATCACGC6550.025.8319573
TATAACG3100.025.7777252
TACATGG184250.025.69092
ACATGGG179200.025.2309253
ACGCAGA238700.024.6029197
CGCAGAG243600.024.0688428
CATGGGG90150.022.93714
TCACGCA7350.022.3785654
Produced by FastQC (version 0.11.5)