FastQCFastQC Report
Mon 6 Jun 2016
ERR523177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909247
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166880.42688527995289116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139260.35623228718983474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84550.2162820614814055No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.1003006461346648No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG38850.043.34592
GGTATCA105350.041.6265951
ACCTGGG45250.036.6602483
GTACCTG52400.033.4940031
GTATCAA168350.033.3439331
TATCACG4200.032.4791562
TATAACG3100.028.830222
GTACATG177650.028.1021611
TCAACGC203600.027.027244
ATCAACG204800.026.869913
CAACGCA207050.026.5768955
ACATGGG180050.026.387443
TACATGG186750.026.145252
CGTACCG909.531422E-426.1065855
AACGCAG213000.026.0109846
TATCAAC213750.025.7915552
CATGGGG91400.024.9355014
ATCACGC5900.024.6915823
ACGCAGA239100.023.1323347
CCTGGGG39650.022.8737144
Produced by FastQC (version 0.11.5)