FastQCFastQC Report
Mon 6 Jun 2016
ERR523176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11869
Sequences flagged as poor quality0
Sequence length100
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTCC250.00510944256.39999894
GCAGAGT459.738281E-441.777789
GAGTACT250.001668618937.610-11
CGCAGAG500.001634964837.68
AGAGTAC352.12137E-433.5714310-11
Produced by FastQC (version 0.11.5)