FastQCFastQC Report
Mon 6 Jun 2016
ERR523176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11869
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG9708.17255034122504TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG7986.723397084842868TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG7976.714971775212739RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG6835.754486477378044RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA2762.325385457915578RNA PCR Primer, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA2752.3169601482854496RNA PCR Primer, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA2181.836717499368102RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA2091.7608897126969416RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG2091.7608897126969416TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG710.5981969837391524RNA PCR Primer, Index 33 (96% over 29bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA610.5139438874378633RNA PCR Primer, Index 3 (96% over 28bp)
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA460.38756424298592973TruSeq Adapter, Index 10 (96% over 28bp)
TCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA420.35386300446541413TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG400.3370123852051563RNA PCR Primer, Index 23 (96% over 30bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA360.30331114668464065RNA PCR Primer, Index 26 (96% over 31bp)
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT330.27803521779425394TruSeq Adapter, Index 4 (96% over 26bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA300.25275928890386723RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG300.25275928890386723RNA PCR Primer, Index 31 (96% over 29bp)
GAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAA260.21905805038335158Illumina PCR Primer Index 9 (96% over 27bp)
GAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAA230.19378212149296486RNA PCR Primer, Index 26 (96% over 31bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT220.18535681186283595No Hit
GAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAA220.18535681186283595TruSeq Adapter, Index 18 (96% over 27bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG220.18535681186283595TruSeq Adapter, Index 11 (96% over 28bp)
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT220.18535681186283595TruSeq Adapter, Index 1 (96% over 27bp)
TCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA200.16850619260257815TruSeq Adapter, Index 4 (96% over 27bp)
GAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAA180.15165557334232033Illumina PCR Primer Index 5 (96% over 27bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG180.15165557334232033RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTTCTGCTTG160.1348049540820625RNA PCR Primer, Index 26 (96% over 29bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT160.1348049540820625TruSeq Adapter, Index 10 (96% over 27bp)
TCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTTCTGCTTG160.1348049540820625Illumina PCR Primer Index 5 (96% over 26bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCTTCTGCTTG160.1348049540820625Illumina PCR Primer Index 8 (96% over 26bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT140.1179543348218047RNA PCR Primer, Index 26 (96% over 30bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGCCTTCTGCTTG130.10952902519167579Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT130.10952902519167579RNA PCR Primer, Index 46 (96% over 26bp)
GAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAA120.10110371556154689Illumina PCR Primer Index 12 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA5200.077.730761
CTCCGAG6950.058.8345342
CCCACGA7200.058.7500049
AGCCCAC7150.058.5034987
GCCCACG7150.058.5034988
TCCGAGC7050.058.03
GAGCCCA7200.057.4444476
CCGAGCC7150.057.1888124
CGAGCCC7250.057.0482755
CTGAGCA900.047.00000420-21
GTAGAGG301.5391288E-647.018-19
ACGTAGA301.5391288E-647.016-17
GCATCTC850.046.99999624-25
TCCTGAG950.044.52631418-19
GAGCATC900.044.3888922-23
GAATCTC1750.044.31428524-25
AGGCGAA1650.044.15151620-21
TAAGGCG1650.044.15151618-19
CGAGACT4450.043.83146312-13
AGACTAG1800.043.08333614-15
Produced by FastQC (version 0.11.5)