FastQCFastQC Report
Mon 6 Jun 2016
ERR523175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5984842
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159300.2661724403083657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129760.21681441214321112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77640.12972773550245773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123600.063.228192
GTACCTG146750.053.461811
ACCTGGG144400.053.439393
CCTGGGG124300.042.9721644
CTGGGGG75650.034.1221285
TGGGGGG118700.029.5343766
TATAACG7150.028.9364242
GGTATCA150450.028.8559421
GTATCAA210650.028.2681881
GTACATG247300.028.2060811
CATGGGG167050.027.5278574
TACATGG255050.027.0459982
ATGGGGG107250.026.9180625
GTACCCG12200.026.2165051
ATAACGC8800.026.1838473
ACATGGG260550.026.0793553
TCAACGC245150.023.7831734
CAACGCA248050.023.5426375
ATCAACG249550.023.365993
TATCAAC252500.023.1477052
Produced by FastQC (version 0.11.5)