FastQCFastQC Report
Mon 6 Jun 2016
ERR523175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5984842
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137980.23054911056966917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119760.20010553327890696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70190.11727962074855108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG126450.062.8520472
ACCTGGG145750.053.297213
GTACCTG150550.053.217011
CCTGGGG131700.040.8563844
CTGGGGG76450.034.4231645
TATAACG6600.034.1815642
GGTATCA148500.029.9836921
TGGGGGG110800.029.9017646
GTACCCG13200.028.1393951
GTATCAA208300.028.0345731
ATAACGC8100.027.8481543
TAACGCA9050.026.4826764
GTACATG248600.025.9864651
CATGGGG168350.025.9603214
ATGGGGG106700.025.5008325
TACCCGG15000.024.753152
TACATGG257800.024.7213082
TATCACG5950.024.4872152
ACATGGG261600.023.6585583
TCAACGC245600.023.401214
Produced by FastQC (version 0.11.5)