FastQCFastQC Report
Mon 6 Jun 2016
ERR523174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8480937
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298400.3518479149178917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254190.29971924092821345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149550.17633664770767665No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114760.135315236983838No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103960.12258079502300277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG127350.049.7012182
GTACCTG144550.044.3208921
ACCTGGG146600.042.340983
CCTGGGG118800.029.5261884
CATGGGG188500.022.4749344
GTACATG430550.021.4022941
TACATGG437900.020.4784322
GTACCCG14700.020.1591741
ACATGGG437750.020.109473
GAGTACT261650.019.93422912-13
GTATCAA516000.019.8179781
ATGGGGG105450.019.4844865
GGTATCA406700.018.93321
TATCACG8350.018.5843942
GGACCGT9800.018.2217756
TAACGCA8300.018.1282884
CTGGGGG84050.018.1243085
TATAACG7850.017.971012
GTACTTT293800.017.5854514-15
TCAACGC570650.017.583674
Produced by FastQC (version 0.11.5)