FastQCFastQC Report
Mon 6 Jun 2016
ERR523174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8480937
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267930.3159202809783872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247810.2921964872513497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147150.17350677171637993No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120420.14198902786331274No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101290.11943255798268516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG130150.050.373292
GTACCTG146050.045.583611
ACCTGGG151700.042.718223
CCTGGGG127900.030.6061674
CTGGGGG81650.021.0647745
CATGGGG187050.020.3247534
GAGTACT263300.019.94129212-13
GTACATG421550.019.7745531
GGTATCA410000.019.3339581
GTATCAA520000.019.2947031
TACATGG429500.019.2036722
GTACCCG14750.019.1252121
ACATGGG422500.018.7193983
ATGGGGG106450.018.7176845
TATCACG7250.018.1505722
GTACTTT293150.017.78851714-15
TCAACGC568950.017.3121764
GTATAGG25000.017.3019431
TAACGCA9250.017.2731554
AGTACTT281400.017.2304312-13
Produced by FastQC (version 0.11.5)