FastQCFastQC Report
Mon 6 Jun 2016
ERR523173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7077160
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235340.3325345194965212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199490.2818786066727331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113010.15968269757925496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159150.039.6860541
GTATCAA250650.034.8427731
TACCTGG71200.030.9787922
TCAACGC295450.029.1135524
CAACGCA299050.028.6668995
ATCAACG302450.028.424393
TATCAAC305150.028.2501492
AACGCAG307950.028.041286
ACGCAGA349400.024.6325727
ACCTGGG87400.023.8375073
CGCAGAG362600.023.6956258
GTACATG269800.023.4972211
TACATGG272900.022.7307052
GTACCTG102600.022.1395261
ACATGGG276400.021.5579343
GCAGAGT392850.021.3199069
GAGTACT200850.021.17781312-13
CATGGGG175800.021.1603644
CCGTATC30850.021.01808794
CGTATCA22850.020.76846994
Produced by FastQC (version 0.11.5)