FastQCFastQC Report
Mon 6 Jun 2016
ERR523173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7077160
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225040.3179806589083757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205510.29038484363784345No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT142640.2015497742032115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114040.1611380836380695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162200.041.725451
GTATCAA252550.034.2472271
TATAACG6400.033.0490952
TACCTGG73000.029.7472042
TCAACGC306500.027.6645664
CAACGCA310800.027.3124545
ATCAACG311800.027.1641753
TATCAAC317100.026.8589462
AACGCAG322600.026.456886
ACCTGGG85650.024.3105163
ACGCAGA364000.023.409377
CGCAGAG376450.022.5970768
ATAACGC8000.022.3260293
GTACATG267350.022.0601461
TACATGG271700.021.3477732
GTACCTG104950.021.0623321
TAACGCA8850.020.7128164
GAGTACT209200.020.70181812-13
GCAGAGT410800.020.4671739
ACATGGG274750.020.0496923
Produced by FastQC (version 0.11.5)