FastQCFastQC Report
Mon 6 Jun 2016
ERR523172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771384
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG34200.039.993112
GTACATG95800.031.6359671
ACCTGGG43300.030.717473
TACATGG97950.030.279122
GTACCTG46500.030.1594981
ATGGGGG52350.028.8145895
CATGGGG78600.028.6376274
ACATGGG104050.028.230913
TGGGGGG46850.027.8777146
GTATCAA53050.025.2825131
CCTGGGG44450.024.3153294
TCAACGC58400.021.9671574
CAACGCA58900.021.6211135
TATAACG2005.9666127E-721.1509972
GTACACG6300.020.9159831
TATCACG2258.003917E-820.8898752
ATCAACG61350.020.7606833
TATCAAC62300.020.6719232
AACGCAG63450.020.510216
GTATAAT10800.019.1729831
Produced by FastQC (version 0.11.5)