FastQCFastQC Report
Mon 6 Jun 2016
ERR523172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771384
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG34600.039.0075262
GTACATG91250.030.624481
ACCTGGG43500.030.5716173
GTACCTG47350.029.409451
TACATGG94550.029.0962472
TGGGGGG41750.028.2507936
ACATGGG99900.026.9061643
CATGGGG78750.025.6585034
TATAACG1859.98989E-925.4197272
GTATCAA53400.025.1964841
ATGGGGG52350.025.1336045
TATCACG2202.4392648E-923.5132472
TCAACGC57600.022.6796174
CAACGCA59050.022.2022865
CCTGGGG44000.022.1070654
ATCAACG60750.021.5040263
AACGCAG62750.021.4173496
AATACCG2206.328082E-821.3594825
TATCAAC61950.021.3308262
TATACCG2251.850698E-618.7963455
Produced by FastQC (version 0.11.5)