FastQCFastQC Report
Mon 6 Jun 2016
ERR523171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955416
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219490.27590009120830383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175060.2200513461521057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104670.13157074375494632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133200.040.471071
GTATCAA223900.036.8291851
TCAACGC266650.030.2791334
CAACGCA270550.029.8764555
ATCAACG270900.029.7882523
TATCAAC277800.029.0853252
AACGCAG280200.028.9935346
GTACATG246150.028.4932581
TACATGG253950.027.1341382
ACATGGG260300.025.9096453
TACCTGG68700.025.7937742
ACGCAGA317950.025.4780647
CGCAGAG332050.024.3794148
CATGGGG189900.023.7084084
GTACCTG84400.022.1822171
GCAGAGT368600.021.5020249
ATGGGGG114950.021.4633985
GAGTACT206700.020.1846312-13
AGAGTAC308150.020.1318410-11
TATCACG6550.020.0931032
Produced by FastQC (version 0.11.5)