FastQCFastQC Report
Mon 6 Jun 2016
ERR523171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955416
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194600.24461322952816045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168060.21125230911871862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98760.12414184248818666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141650.040.1844641
GTATCAA228000.035.4262241
TCAACGC265900.029.8513324
CAACGCA271550.029.1956215
ATCAACG272900.029.0517393
AACGCAG281250.028.372146
TATCAAC281800.028.2527392
GTACATG242700.026.457711
TACCTGG67050.025.7401962
TACATGG250000.025.3568022
ACGCAGA321600.024.7533177
ACATGGG259500.023.579483
CGCAGAG337450.023.5682878
CATGGGG184650.022.0659284
GTACCTG83350.021.1648451
GCAGAGT377750.020.867279
GAGTACT213900.020.26133512-13
ATGGGGG109350.019.8122845
AGAGTAC315150.019.36006210-11
ACCTGGG89350.018.356573
Produced by FastQC (version 0.11.5)