##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523171_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7955416 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.605992823002595 34.0 31.0 34.0 30.0 35.0 2 32.67690300544937 34.0 31.0 34.0 30.0 34.0 3 32.94325966109126 34.0 31.0 34.0 31.0 36.0 4 35.73318089211174 37.0 35.0 37.0 32.0 37.0 5 35.79673306839014 37.0 35.0 37.0 35.0 37.0 6 35.8735919026736 37.0 35.0 37.0 35.0 37.0 7 35.69874548357999 37.0 35.0 37.0 35.0 37.0 8 35.74431997019389 37.0 35.0 37.0 33.0 37.0 9 36.95678076419888 39.0 35.0 39.0 34.0 39.0 10-11 37.07102720963932 38.0 36.0 39.0 34.0 39.0 12-13 36.93694258100393 38.0 36.0 39.0 33.5 39.0 14-15 38.01950444829032 39.0 36.5 41.0 34.5 41.0 16-17 38.039292539824444 39.0 37.0 41.0 34.0 41.0 18-19 38.03619389608287 39.0 37.0 41.0 34.0 41.0 20-21 37.918730082751175 39.0 36.5 41.0 34.0 41.0 22-23 37.83107464147695 39.0 36.5 41.0 34.0 41.0 24-25 37.691350647156604 39.0 36.0 41.0 33.5 41.0 26-27 37.56131112439626 38.5 36.5 41.0 33.0 41.0 28-29 37.52033746569633 38.0 36.5 41.0 33.5 41.0 30-31 37.363806242187714 38.0 36.0 41.0 33.5 41.0 32-33 37.21476576460615 38.0 36.5 41.0 32.5 41.0 34-35 37.03252155512672 38.0 36.0 40.0 32.0 41.0 36-37 37.050491388508156 38.0 35.5 40.0 33.0 41.0 38-39 37.18535699453052 38.0 36.0 40.0 32.0 41.0 40-41 37.087603589805994 38.0 36.5 40.0 32.5 41.0 42-43 37.11663217863151 38.0 36.5 40.5 32.5 41.0 44-45 36.94773246301639 38.0 35.5 41.0 32.0 41.0 46-47 36.69498779196462 38.0 36.0 40.5 31.5 41.0 48-49 36.65437734494337 38.0 36.0 40.0 31.0 41.0 50-51 36.26324575609874 37.5 35.0 40.0 30.5 41.0 52-53 36.30396059740936 38.0 35.0 40.0 30.5 41.0 54-55 36.10557480589324 37.5 35.0 40.0 30.5 41.0 56-57 35.80257858294274 37.0 35.0 40.0 29.0 41.0 58-59 35.66904464832511 36.5 35.0 40.0 29.0 41.0 60-61 35.39325410512788 36.5 35.0 40.0 28.5 41.0 62-63 35.197235820226126 37.5 34.5 39.0 27.5 41.0 64-65 35.05674505267858 37.0 34.0 39.0 28.0 41.0 66-67 34.82782364869418 36.5 34.0 39.0 28.0 40.5 68-69 34.357668788156396 36.0 34.0 38.0 26.5 40.0 70-71 34.109285674061546 36.0 34.0 37.5 26.0 40.0 72-73 33.84675182290907 36.0 33.5 37.0 26.0 39.0 74-75 33.56326696177799 35.5 33.0 37.0 26.0 39.0 76-77 32.09772097398803 34.0 31.0 36.0 24.5 37.5 78-79 33.1158101600218 35.0 32.5 37.0 26.0 37.0 80-81 33.12218085138477 35.0 33.0 36.5 26.0 37.0 82-83 32.94029124309779 35.0 33.0 36.0 25.5 37.5 84-85 32.739013145258525 35.0 33.0 36.0 25.0 37.0 86-87 32.49294926877488 35.0 33.0 36.0 25.0 37.0 88-89 32.332018966701426 35.0 32.5 36.0 24.5 36.5 90-91 32.18792084537125 35.0 32.0 35.5 24.0 37.0 92-93 32.0312812680066 35.0 32.0 35.5 23.5 36.5 94-95 31.90774932448536 35.0 32.0 35.0 23.5 36.0 96-97 31.724051074136163 35.0 31.0 35.0 23.0 36.5 98-99 31.585604699993063 34.5 31.0 35.0 22.0 36.5 100 30.378041450000854 34.0 29.0 35.0 19.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0710944513534173 1101 2 -0.10533046065076235 1101 3 -0.13104485697279955 1101 4 -0.11136282381556128 1101 5 -0.12203435164114751 1101 6 -0.2161125444248313 1101 7 -0.15751191966128886 1101 8 -0.11673587596470014 1101 9 -0.17413770082571034 1101 10-11 -0.13535257862839956 1101 12-13 -0.18377231878258726 1101 14-15 -0.3649465363660198 1101 16-17 -0.17893754205518775 1101 18-19 -0.21707967492370983 1101 20-21 -0.25261936750511893 1101 22-23 -0.1580628841540488 1101 24-25 -0.2726327552161223 1101 26-27 -0.30495745079605996 1101 28-29 -0.3323418281709891 1101 30-31 -0.3335090790633046 1101 32-33 -0.2610268974086907 1101 34-35 -0.20449573344903627 1101 36-37 -0.4091503702797823 1101 38-39 -0.32365195159740523 1101 40-41 -0.3206174359289591 1101 42-43 -0.3494236668277466 1101 44-45 -0.36321555826405216 1101 46-47 -0.33155614801577116 1101 48-49 -0.55350619750525 1101 50-51 -0.36835400071681335 1101 52-53 -0.4300671373433502 1101 54-55 -0.4193366125858091 1101 56-57 -0.43286268760185465 1101 58-59 -0.5817232289182428 1101 60-61 -0.5465551138547653 1101 62-63 -0.4478613613274689 1101 64-65 -0.41279076033134743 1101 66-67 -0.5639023495123467 1101 68-69 -0.4977959589814773 1101 70-71 -0.5681387926436727 1101 72-73 -0.7195262193410059 1101 74-75 -0.670947254688123 1101 76-77 -0.5355304590817553 1101 78-79 -0.483885437078591 1101 80-81 -0.3923005795937584 1101 82-83 -0.5115851432693432 1101 84-85 -0.4397958070005856 1101 86-87 -0.4662464843813652 1101 88-89 -0.48505924540693357 1101 90-91 -0.5843489872611585 1101 92-93 -0.8431411806695976 1101 94-95 -0.7131248856595143 1101 96-97 -0.6621406546754827 1101 98-99 -0.619316731172912 1101 100 -0.6464229758889992 1102 1 -0.018936780349939397 1102 2 -0.0550753586099475 1102 3 -0.1553254416142309 1102 4 -0.05472223475757687 1102 5 -0.0930934966697663 1102 6 -0.10228677290792376 1102 7 -0.11894677087860117 1102 8 -0.046169682850845106 1102 9 -0.16333370384369061 1102 10-11 -0.13408856986889361 1102 12-13 -0.1353150075442997 1102 14-15 -0.22746689392293007 1102 16-17 -0.15929037187061823 1102 18-19 -0.16962810750683843 1102 20-21 -0.21316607717184866 1102 22-23 -0.15738015840369712 1102 24-25 -0.2148185012458228 1102 26-27 -0.18789666796173776 1102 28-29 -0.19189757337258584 1102 30-31 -0.1808544881466858 1102 32-33 -0.1808142126954877 1102 34-35 -0.20226198038965038 1102 36-37 -0.2290899646928395 1102 38-39 -0.23267529492932937 1102 40-41 -0.24784923374276957 1102 42-43 -0.19912899981757448 1102 44-45 -0.3191083233953478 1102 46-47 -0.2742472675203871 1102 48-49 -0.2260807728719172 1102 50-51 -0.16510467010088803 1102 52-53 -0.205143794471482 1102 54-55 -0.21586760437838137 1102 56-57 -0.16398599918979073 1102 58-59 -0.33252039404218436 1102 60-61 -0.33811058072139843 1102 62-63 -0.2276571186745997 1102 64-65 -0.269794680023665 1102 66-67 -0.3693607625890323 1102 68-69 -0.3831674441400068 1102 70-71 -0.3837271605426835 1102 72-73 -0.49721203667965597 1102 74-75 -0.8453643614767401 1102 76-77 -0.40772031112717144 1102 78-79 -0.41683184264712736 1102 80-81 -0.42549254100411815 1102 82-83 -0.3870466387331746 1102 84-85 -0.3846832423624704 1102 86-87 -0.4273376724345894 1102 88-89 -0.44920400948929995 1102 90-91 -0.4265613591350643 1102 92-93 -0.4619537485281242 1102 94-95 -0.5308654038686029 1102 96-97 -0.6111086300531916 1102 98-99 -0.7297480238432748 1102 100 -0.44525776453845367 1103 1 -0.1536927797057146 1103 2 -0.11757075699478037 1103 3 -0.12292395133002998 1103 4 -0.04772322602217827 1103 5 -0.1361728591926763 1103 6 -0.05872665344488581 1103 7 -0.07195483712652617 1103 8 -0.05288986246722516 1103 9 -0.10377269793061572 1103 10-11 -0.12263454256085993 1103 12-13 -0.10040576481676311 1103 14-15 -0.23514735712662116 1103 16-17 -0.19107626031468072 1103 18-19 -0.17654880090633185 1103 20-21 -0.16057957000227674 1103 22-23 -0.13420770264950477 1103 24-25 -0.12163733597625992 1103 26-27 -0.18988916732901373 1103 28-29 -0.17139876787518915 1103 30-31 -0.16116535121796005 1103 32-33 -0.10850011794823189 1103 34-35 -0.11821860026044817 1103 36-37 -0.1215307572443649 1103 38-39 -0.17749959631467505 1103 40-41 -0.2685773131180724 1103 42-43 -0.3264605167911441 1103 44-45 -0.40577001456561845 1103 46-47 -0.3537455780245935 1103 48-49 -0.32863000376307383 1103 50-51 -0.18423963195861148 1103 52-53 -0.20035614143582592 1103 54-55 -0.24469871263904253 1103 56-57 -0.08718410735075821 1103 58-59 -0.23934380642104003 1103 60-61 -0.35046694801464895 1103 62-63 -0.2543962256289163 1103 64-65 -0.36727823564057616 1103 66-67 -0.3456238500299449 1103 68-69 -0.19051349934149897 1103 70-71 -0.3927349180989168 1103 72-73 -0.42337253846022804 1103 74-75 -0.397931423626666 1103 76-77 -0.25239967761258697 1103 78-79 -0.2932928634515406 1103 80-81 -0.2522082324479271 1103 82-83 -0.19831878240838563 1103 84-85 -0.22928328690866095 1103 86-87 -0.24609291316297544 1103 88-89 -0.27728556576833086 1103 90-91 -0.2849252101245412 1103 92-93 -0.3226567698719478 1103 94-95 -0.06339086613919065 1103 96-97 -0.7638063109669702 1103 98-99 -1.1350756955921675 1103 100 -0.2695559702920072 1104 1 -0.016066648839832 1104 2 -0.09537249337495268 1104 3 -0.06908492539258759 1104 4 -0.08460700878336525 1104 5 -0.11427588931365307 1104 6 -0.08598580404203915 1104 7 -0.17460754604862672 1104 8 -0.06834448976060514 1104 9 -0.0673261683632802 1104 10-11 -0.10063264109000869 1104 12-13 -0.07557662646134133 1104 14-15 -0.2109020930436145 1104 16-17 -0.07029488085314739 1104 18-19 -0.124213815543051 1104 20-21 -0.2234745158017546 1104 22-23 -0.13989598832450412 1104 24-25 -0.1049107957403379 1104 26-27 -0.06616464139684552 1104 28-29 -0.19693439710574268 1104 30-31 -0.3218846104178468 1104 32-33 -0.21123243314695372 1104 34-35 -0.14939404747965312 1104 36-37 -0.26973049985627284 1104 38-39 -0.305304511409453 1104 40-41 -0.2380977979683081 1104 42-43 -0.19307867780721466 1104 44-45 -0.2945906785422281 1104 46-47 -0.23692503199023207 1104 48-49 -0.30444403508550266 1104 50-51 -0.23833043202454718 1104 52-53 -0.22384726951599987 1104 54-55 -0.2813744276089949 1104 56-57 -0.30876292261859106 1104 58-59 -0.2821494024203801 1104 60-61 -0.2980101844257419 1104 62-63 -0.15908671128446628 1104 64-65 -0.3074876736246921 1104 66-67 -0.3431187951302448 1104 68-69 -0.23398074095134547 1104 70-71 -0.22908033464475608 1104 72-73 -0.0748566707424061 1104 74-75 -0.21528841393647014 1104 76-77 -0.26612181448643923 1104 78-79 -0.22783722140552953 1104 80-81 -0.08650569942444974 1104 82-83 -0.052762498097500554 1104 84-85 -0.10830575093781647 1104 86-87 -0.18325287315308714 1104 88-89 -0.16784602037245833 1104 90-91 -0.15836039709852656 1104 92-93 -0.28692757471726793 1104 94-95 -0.20706985016284207 1104 96-97 -0.038063419103551155 1104 98-99 -0.9574880997006154 1104 100 -0.27519005409779496 1105 1 -0.087383417511667 1105 2 -1.0186838670209255 1105 3 -0.16386799144269304 1105 4 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0.0028503075718973037 1106 18-19 -0.027096064201714398 1106 20-21 -0.09551114934706106 1106 22-23 0.04855563054602641 1106 24-25 0.014227354901514389 1106 26-27 0.05840746344691894 1106 28-29 -0.006012182630918517 1106 30-31 -0.0022535050705272397 1106 32-33 0.07016264146080431 1106 34-35 0.07634833568799593 1106 36-37 0.004875938741704999 1106 38-39 -0.021805459190154863 1106 40-41 0.017153566157681155 1106 42-43 0.019841881217772084 1106 44-45 -0.09566693464473275 1106 46-47 -0.04207174487229537 1106 48-49 -0.058522286062562046 1106 50-51 -0.017959013057577522 1106 52-53 -0.02350958591001273 1106 54-55 -0.00609750731516101 1106 56-57 0.07671055382166259 1106 58-59 -0.026401056063086514 1106 60-61 0.04817768458472216 1106 62-63 0.03500154235078412 1106 64-65 0.0502277191799152 1106 66-67 0.07211665154736124 1106 68-69 0.07828570419250269 1106 70-71 0.023372142593203193 1106 72-73 0.02376855067723227 1106 74-75 0.0301956186128578 1106 76-77 -0.018649255875800463 1106 78-79 0.014404157850634647 1106 80-81 0.15027743161908091 1106 82-83 0.10275705713541328 1106 84-85 0.19431905977789654 1106 86-87 0.12525379098004663 1106 88-89 0.11737295666301151 1106 90-91 0.09650359855532287 1106 92-93 0.048085450078147574 1106 94-95 0.06646535770432038 1106 96-97 -5.094513573205006E-4 1106 98-99 -0.005154009408229854 1106 100 -0.039107316554382265 1107 1 -0.05844806778455336 1107 2 -0.08423015520540389 1107 3 -0.05138157910977981 1107 4 -0.0494880662560675 1107 5 -0.06437298167585936 1107 6 -0.1129493046938066 1107 7 -0.11251771670593769 1107 8 -0.031883655094944174 1107 9 -0.13796819760187162 1107 10-11 -0.11091027053244318 1107 12-13 -0.08238729000851919 1107 14-15 -0.13845154767629708 1107 16-17 -0.08772335633252482 1107 18-19 -0.08181540531856513 1107 20-21 -0.1305111664732408 1107 22-23 -0.0203177000952266 1107 24-25 0.026642899915771068 1107 26-27 -0.0777091375551251 1107 28-29 -0.13913869088127484 1107 30-31 -0.084626177149147 1107 32-33 0.00499668453142732 1107 34-35 -0.01901550442153166 1107 36-37 -0.1031447266812009 1107 38-39 -0.05916584568391414 1107 40-41 -0.05750911545165849 1107 42-43 -0.09209102980036477 1107 44-45 -0.09105916750787202 1107 46-47 -0.047628241008325745 1107 48-49 -0.10442612674214757 1107 50-51 -0.01859508230165119 1107 52-53 -0.21911733946824796 1107 54-55 -0.1305872084279116 1107 56-57 0.0623042284397215 1107 58-59 -0.15273235631541837 1107 60-61 -0.07224439675702854 1107 62-63 5.272682529451345E-4 1107 64-65 -0.10935461803180146 1107 66-67 -0.10547696410773 1107 68-69 -0.08724671009313312 1107 70-71 -0.21967623542946058 1107 72-73 -0.2759335547781774 1107 74-75 -0.23395580198511112 1107 76-77 -0.30841837789381543 1107 78-79 -0.15643721815933986 1107 80-81 -0.021426033287731627 1107 82-83 -0.06944389260892336 1107 84-85 0.01718242249526014 1107 86-87 0.03609367884762804 1107 88-89 0.028398373009530076 1107 90-91 -0.04904463236571388 1107 92-93 0.13011567508797128 1107 94-95 0.04375222519334443 1107 96-97 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0.23847443852877603 2315 88-89 0.152848791092687 2315 90-91 0.1985056081617671 2315 92-93 0.10830807433132605 2315 94-95 0.27202599348354184 2315 96-97 0.20907080857778837 2315 98-99 0.30710404347090403 2315 100 0.17987556783132064 2316 1 0.007323602905259463 2316 2 -0.04762834699009488 2316 3 -0.01252990802947096 2316 4 -0.009579086824828664 2316 5 -0.017704014452007755 2316 6 -4.484080282978198E-4 2316 7 -0.021727118769057086 2316 8 0.03630200185066457 2316 9 -0.018466292284941233 2316 10-11 -0.012981776547078994 2316 12-13 -0.0052607708237033535 2316 14-15 -0.02345112958609974 2316 16-17 0.012787520167030664 2316 18-19 0.05440901813788912 2316 20-21 0.030638556080418766 2316 22-23 0.09796156377740317 2316 24-25 0.026043389710906695 2316 26-27 -1.5337897747726785 2316 28-29 -0.015661349176518513 2316 30-31 0.004984154305105903 2316 32-33 -0.05091499048501902 2316 34-35 0.11540368907329679 2316 36-37 0.018870834480019028 2316 38-39 -0.3623618586739994 2316 40-41 -1.5190555638568455 2316 42-43 -0.11778904587399097 2316 44-45 -0.25512463406469266 2316 46-47 -0.06749870237756994 2316 48-49 -0.162499710049552 2316 50-51 -0.3149297188595952 2316 52-53 -0.28740381203636645 2316 54-55 -0.1814856283582671 2316 56-57 -0.14435845257528968 2316 58-59 -0.26005621406967094 2316 60-61 -0.1853409905882799 2316 62-63 -1.310462257137587 2316 64-65 -0.23192091220911237 2316 66-67 -0.14501926217193528 2316 68-69 -0.029347705968397975 2316 70-71 0.04090171518722485 2316 72-73 0.10080383633621182 2316 74-75 -0.044796880598376276 2316 76-77 -0.18935949961776544 2316 78-79 -0.19733889650493808 2316 80-81 -0.1166804691114578 2316 82-83 -0.12247406315717768 2316 84-85 0.022102332402845093 2316 86-87 -0.23992468910653741 2316 88-89 -0.09122399321682195 2316 90-91 -0.08660961850790017 2316 92-93 -0.09380588921168709 2316 94-95 -0.02805281942204374 2316 96-97 0.020612067725057415 2316 98-99 0.06646799926717861 2316 100 -0.21025716239777026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 1.0 11 9.0 12 27.0 13 167.0 14 636.0 15 1818.0 16 3993.0 17 7762.0 18 12934.0 19 19452.0 20 26637.0 21 36642.0 22 47690.0 23 55260.0 24 60619.0 25 66865.0 26 76543.0 27 91146.0 28 110439.0 29 135406.0 30 167902.0 31 210104.0 32 268930.0 33 360443.0 34 542135.0 35 1025484.0 36 1698926.0 37 1313139.0 38 1348715.0 39 265465.0 40 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.988025542290835 18.764296621319847 13.122915233232337 24.124762603156977 2 16.39017488175106 20.63460754913836 39.084523475123035 23.89069409398754 3 19.14060629174857 25.481065057591795 29.811508503920635 25.566820146739 4 13.349925805253404 16.66141866891922 37.09863030401305 32.89002522181433 5 13.826200438399447 37.12765398472616 34.373553637561464 14.672591939312927 6 31.55333661079932 36.24577374699462 17.881363357134138 14.319526285071912 7 27.62047892907172 32.05926629103997 22.177256349636522 18.142998430251794 8 25.98767073971539 34.8349997938503 20.426405563829476 18.75092390260483 9 26.716542147659982 15.619455032624332 20.10379852105711 37.560204298658576 10-11 25.15755308271826 25.587342154663478 28.46379207920543 20.79131268341283 12-13 25.661922076563858 23.36990361843633 28.274759891427365 22.693414413572448 14-15 23.03188665790224 24.912997387328748 25.89824442194845 26.156871532820563 16-17 22.8769541842589 27.60930135738495 26.534368420883926 22.979376037472218 18-19 22.70714547485983 26.810185854134023 28.206752000259446 22.2759166707467 20-21 23.434640405596173 26.106818883599892 28.088994388151793 22.36954632265214 22-23 23.185616378475125 25.945579097530374 27.984196137642336 22.884608386352166 24-25 23.01109277127713 26.01618581482349 28.027181921725486 22.945539492173893 26-27 22.90975196093486 26.139619454404695 28.074145779999547 22.8764828046609 28-29 22.880317062174125 26.341864492678337 28.051528260270665 22.72629018487688 30-31 23.351003969010243 26.069547186870334 27.97543140923749 22.604017434881932 32-33 23.07552525731183 26.206046956034086 27.93439699427879 22.784030792375294 34-35 23.285125866810777 26.257264919069794 27.868125530359755 22.589483683759674 36-37 23.23742228522558 26.2030992196672 27.784996388808906 22.774482106298304 38-39 23.11835076837521 26.36636648338812 27.768538183631065 22.74674456460561 40-41 23.16474535125431 26.20621510490613 27.838000217091157 22.791039326748404 42-43 23.04119509708209 26.30058048696696 27.63847413238225 23.01975028356869 44-45 22.8856977211348 26.41183947931191 27.691843833799375 23.010618965753917 46-47 22.96916628101102 26.275627556343757 27.42678608853648 23.328420074108745 48-49 23.035512823486687 26.38562345787159 27.376231651850354 23.202632066791374 50-51 22.96884075280037 26.599317350363954 27.304445394315024 23.12739650252065 52-53 23.036340829051895 26.650360339697404 27.112748671852938 23.20055015939776 54-55 22.875068491842008 26.435808358349068 27.363174386169163 23.325948763639758 56-57 22.96946057849268 26.578685508117555 27.113824296129362 23.338029617260403 58-59 22.964855224401422 26.4581894024644 27.25889128228954 23.31806409084464 60-61 23.092928648534837 26.496020665825494 27.28972538273102 23.121325302908645 62-63 22.88840170160644 26.56798767244799 27.256274317069924 23.28733630887565 64-65 22.813356385120724 26.341217615321632 27.25925397964823 23.586172019909412 66-67 22.76691279436647 26.42199712892918 27.097899241001507 23.713190835702846 68-69 22.638922193137965 26.480952047223976 27.172340925026 23.70778483461206 70-71 22.729605051368324 26.268948004930998 27.151770956769056 23.849675986931626 72-73 22.698157314037502 26.448934496439858 26.93215150487403 23.92075668464861 74-75 22.687467075876476 26.537567960953602 26.85959189517682 23.915373067993105 76-77 22.66491914627863 26.367773384184233 26.90261418431844 24.064693285218695 78-79 22.593829493349396 26.31918419804834 26.951372382989057 24.135613925613207 80-81 22.651403882313435 26.516672259314145 26.66141068839467 24.17051316997775 82-83 22.77074335161879 26.411099742059402 26.727466651247216 24.09069025507459 84-85 22.723198853601666 26.430207344742847 26.613983042857953 24.232610758797538 86-87 22.51800885734159 26.43477702315362 26.80518317204402 24.242030947460766 88-89 22.444638262630374 26.24304452740623 26.801405572221864 24.510911637741533 90-91 22.715466188580937 26.180655440451144 26.669515906773285 24.434362464194635 92-93 22.64519199785152 26.171858231687867 26.642752896354466 24.540196874106147 94-95 22.53428921920777 25.999186961517562 26.69182837245358 24.774695446821084 96-97 22.468456365799497 25.908905867418593 26.817417339239487 24.805220427542427 98-99 22.451649536036168 25.856917019499075 26.697838103788417 24.993595340676336 100 22.446460583980418 25.86555645882786 26.720638408895407 24.967344548296317 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1416.0 1 1063.0 2 1395.0 3 1930.5 4 1364.0 5 1106.0 6 1623.5 7 3006.5 8 4743.5 9 5675.0 10 5286.5 11 4807.5 12 5141.0 13 5657.0 14 6282.0 15 6795.5 16 7307.5 17 8071.0 18 8815.0 19 9563.0 20 10526.5 21 11940.0 22 13941.5 23 16588.0 24 20368.0 25 25522.5 26 32207.5 27 40546.0 28 50361.5 29 61331.5 30 73410.5 31 85899.5 32 99782.0 33 114491.5 34 128272.0 35 141919.5 36 159958.0 37 178517.5 38 193076.0 39 205900.5 40 216299.0 41 226628.0 42 238351.0 43 252427.0 44 265862.5 45 278295.0 46 290568.0 47 303347.5 48 318802.0 49 327674.5 50 326583.5 51 322871.0 52 318003.0 53 312205.5 54 303325.0 55 285368.0 56 259873.0 57 233522.5 58 208969.5 59 182005.0 60 153055.0 61 126922.0 62 104083.0 63 81764.5 64 61877.0 65 47056.0 66 35326.0 67 27458.5 68 21308.0 69 15991.0 70 12513.5 71 9869.0 72 7992.0 73 6544.0 74 5172.0 75 4014.0 76 2900.0 77 1884.0 78 1243.5 79 834.0 80 559.5 81 385.0 82 273.0 83 168.0 84 105.0 85 83.0 86 55.5 87 36.0 88 22.0 89 12.5 90 9.0 91 5.0 92 4.5 93 3.5 94 2.5 95 2.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0312742916272386 2 0.0738239207101175 3 0.002551720739682249 4 1.2570052904838666E-5 5 0.0014329860311516078 6 0.0010433143911016093 7 0.0 8 4.022416929548373E-4 9 5.028021161935466E-5 10-11 2.514010580967733E-5 12-13 5.656523807177399E-5 14-15 0.011482743328570121 16-17 0.0010370293646491898 18-19 1.31985555500806E-4 20-21 1.9483582002499932E-4 22-23 0.001828942697654026 24-25 6.913529097661266E-4 26-27 0.007548316769355619 28-29 0.007988268621024973 30-31 0.009905201689012868 32-33 0.00904415306503142 34-35 0.004028701956000792 36-37 0.0035761800514266004 38-39 0.0027716966655169256 40-41 0.00292253730037499 42-43 0.00141413095179435 44-45 0.005078301373554821 46-47 0.017962605601014453 48-49 0.01903734512437816 50-51 0.014028179041799952 52-53 0.007925418356500779 54-55 0.0011187347085306414 56-57 0.0023883100519193467 58-59 0.0026145710042064425 60-61 0.0031865084113766014 62-63 0.0028596870358507964 64-65 0.0035761800514266004 66-67 0.002463730369348378 68-69 0.007020374547352395 70-71 3.39391428430644E-4 72-73 0.00500288105612579 74-75 0.007196355288020137 76-77 0.006605562801492719 78-79 0.0043743784108838555 80-81 3.833866135975793E-4 82-83 0.001206725078864512 84-85 8.29623491719352E-4 86-87 0.001187869999507254 88-89 9.993192059346739E-4 90-91 9.867491530298354E-4 92-93 3.456764548830633E-4 94-95 9.176138620532226E-4 96-97 0.002583145871944346 98-99 0.00339391428430644 100 0.004889750579982241 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7955416.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.48932565454457 #Duplication Level Percentage of deduplicated Percentage of total 1 73.63446502961025 41.59561274455828 2 15.206544458257076 17.180148839655885 3 4.949425348395355 8.387691009250888 4 2.152011629246146 4.862627429474104 5 1.1844313594271694 3.3453864389068158 6 0.6773160826735154 2.295667725912279 7 0.43266300092670684 1.7108588810614889 8 0.3137617234452194 1.4179350538902522 9 0.22779578717238014 1.1581227363882518 >10 1.1316518233825525 11.25340876944603 >50 0.060198999291276624 2.3154749791460323 >100 0.027934360281759 2.715989396506164 >500 0.0012002722175998776 0.4280114730154683 >1k 5.053689877946355E-4 0.5097772340090801 >5k 3.158556172807111E-5 0.17621224186164886 >10k+ 6.317112345614222E-5 0.6470750469172294 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19460 0.24461322952816045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16806 0.21125230911871862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9876 0.12414184248818666 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2570052904838666E-5 2 0.0 0.0 0.0 0.0 1.2570052904838666E-5 3 0.0 0.0 0.0 0.0 1.2570052904838666E-5 4 0.0 0.0 0.0 0.0 1.2570052904838666E-5 5 0.0 0.0 0.0 0.0 1.2570052904838666E-5 6 2.514010580967733E-5 0.0 0.0 0.0 5.028021161935466E-5 7 2.514010580967733E-5 0.0 0.0 0.0 5.028021161935466E-5 8 2.514010580967733E-5 0.0 0.0 0.0 5.028021161935466E-5 9 2.514010580967733E-5 0.0 0.0 0.0 6.285026452419333E-5 10-11 2.514010580967733E-5 0.0 0.0 1.2570052904838666E-5 6.285026452419333E-5 12-13 2.514010580967733E-5 0.0 0.0 1.2570052904838666E-5 6.285026452419333E-5 14-15 2.514010580967733E-5 0.0 0.0 1.2570052904838666E-5 1.4455560840564464E-4 16-17 3.7710158714516E-5 0.0 0.0 2.514010580967733E-5 1.759807406677413E-4 18-19 3.7710158714516E-5 0.0 0.0 5.028021161935466E-5 2.2626095228709599E-4 20-21 3.7710158714516E-5 0.0 0.0 6.285026452419333E-5 2.2626095228709599E-4 22-23 3.7710158714516E-5 0.0 0.0 2.45116031644354E-4 2.3883100519193467E-4 24-25 3.7710158714516E-5 0.0 0.0 7.102079891233846E-4 2.514010580967733E-4 26-27 3.7710158714516E-5 0.0 0.0 0.0016341068776290265 2.514010580967733E-4 28-29 3.7710158714516E-5 0.0 0.0 0.00461320941607579 2.514010580967733E-4 30-31 3.7710158714516E-5 0.0 0.0 0.015775416395572527 2.514010580967733E-4 32-33 3.7710158714516E-5 1.2570052904838666E-5 0.0 0.04559786691230226 2.7654116390645063E-4 34-35 6.285026452419333E-5 1.2570052904838666E-5 0.0 0.09413084117788434 2.891112168112893E-4 36-37 6.285026452419333E-5 1.2570052904838666E-5 0.0 0.16863982977131553 2.953962432637086E-4 38-39 6.285026452419333E-5 1.2570052904838666E-5 0.0 0.2744042549126281 3.01681269716128E-4 40-41 6.285026452419333E-5 1.2570052904838666E-5 0.0 0.38647884661216964 3.0796629616854733E-4 42-43 6.285026452419333E-5 1.2570052904838666E-5 0.0 0.5012371446068942 3.268213755258053E-4 44-45 6.285026452419333E-5 1.2570052904838666E-5 0.0 0.6486335849690325 3.456764548830633E-4 46-47 6.285026452419333E-5 1.2570052904838666E-5 0.0 0.8228419481771916 3.6453153424032133E-4 48-49 6.285026452419333E-5 1.2570052904838666E-5 0.0 1.0024114389492642 4.1481174585967595E-4 50-51 7.5420317429032E-5 1.2570052904838666E-5 0.0 1.1873609123646078 4.1481174585967595E-4 52-53 7.5420317429032E-5 1.2570052904838666E-5 0.0 1.3748243963609195 4.3995185166935326E-4 54-55 8.170534388145133E-5 1.2570052904838666E-5 0.0 1.5903505234672832 4.6509195747903063E-4 56-57 8.799037033387066E-5 1.2570052904838666E-5 0.0 1.8440016210340227 4.6509195747903063E-4 58-59 9.427539678629E-5 1.2570052904838666E-5 0.0 2.120555354993378 4.6509195747903063E-4 60-61 1.3827058195322532E-4 1.2570052904838666E-5 0.0 2.406850879953984 4.6509195747903063E-4 62-63 1.50840634858064E-4 1.2570052904838666E-5 0.0 2.6968608555479685 5.27942222003224E-4 64-65 1.50840634858064E-4 1.8855079357257997E-5 0.0 3.001546367908353 5.405122749080626E-4 66-67 1.50840634858064E-4 2.514010580967733E-5 0.0 3.34250276792565 5.405122749080626E-4 68-69 1.50840634858064E-4 2.514010580967733E-5 0.0 3.729082174961058 5.405122749080626E-4 70-71 1.50840634858064E-4 2.514010580967733E-5 0.0 4.142932311773514 5.907924865274173E-4 72-73 1.6969571421532198E-4 2.514010580967733E-5 0.0 4.547776005679653 6.536427510516106E-4 74-75 1.759807406677413E-4 2.514010580967733E-5 0.0 4.95887330090595 6.536427510516106E-4 76-77 1.759807406677413E-4 2.514010580967733E-5 0.0 5.401841965272464 6.536427510516106E-4 78-79 1.759807406677413E-4 2.514010580967733E-5 0.0 5.893639000147823 6.536427510516106E-4 80-81 1.759807406677413E-4 2.514010580967733E-5 0.0 6.406151482210358 6.787828568612879E-4 82-83 1.759807406677413E-4 2.514010580967733E-5 0.0 6.927701832311472 7.5420317429032E-4 84-85 1.759807406677413E-4 2.514010580967733E-5 0.0 7.453701981140899 7.5420317429032E-4 86-87 1.759807406677413E-4 2.514010580967733E-5 0.0 7.993610893509528 7.667732271951586E-4 88 1.759807406677413E-4 2.514010580967733E-5 0.0 8.415474439048818 7.793432800999973E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14165 0.0 40.184464 1 GTATCAA 22800 0.0 35.426224 1 TCAACGC 26590 0.0 29.851332 4 CAACGCA 27155 0.0 29.195621 5 ATCAACG 27290 0.0 29.051739 3 AACGCAG 28125 0.0 28.37214 6 TATCAAC 28180 0.0 28.252739 2 GTACATG 24270 0.0 26.45771 1 TACCTGG 6705 0.0 25.740196 2 TACATGG 25000 0.0 25.356802 2 ACGCAGA 32160 0.0 24.753317 7 ACATGGG 25950 0.0 23.57948 3 CGCAGAG 33745 0.0 23.568287 8 CATGGGG 18465 0.0 22.065928 4 GTACCTG 8335 0.0 21.164845 1 GCAGAGT 37775 0.0 20.86727 9 GAGTACT 21390 0.0 20.261335 12-13 ATGGGGG 10935 0.0 19.812284 5 AGAGTAC 31515 0.0 19.360062 10-11 ACCTGGG 8935 0.0 18.35657 3 >>END_MODULE