FastQCFastQC Report
Mon 6 Jun 2016
ERR523170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6266925
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT491330.7840049146910169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178350.2845893320887038No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT140670.2244641510788784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140180.2236822684171264No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC106910.1705940313630688No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC101980.162727334378503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79150.12629798505646708No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG78250.1248618740450859No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA69920.11156986879530231No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC63950.1020436657531405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG222500.028.7156851
TACATGG230100.027.4570062
TACCTGG49950.025.974322
ACATGGG236950.025.8517283
CATGGGG163150.025.2100774
ATGGGGG101850.023.7241175
GTACCTG60650.021.7823771
GAGTACT159350.020.0791212-13
ACCTGGG66000.019.089423
AGAGTAC260350.018.37899610-11
TGGGGGG100850.018.309296
TATAACG4900.018.2275412
GTACTTT178550.017.80082714-15
GAGTACA168300.017.794471
TATACCG4800.017.6286565
AGTACTT171750.017.04279912-13
AGTACAT169150.016.6188032
GTATAGC20800.015.370051
CATGGGA80250.015.053634
ACTTTTT220650.014.63777116-17
Produced by FastQC (version 0.11.5)