FastQCFastQC Report
Mon 6 Jun 2016
ERR523170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6266925
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT508410.8112591103292285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168420.2687442405964648No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT145200.23169257650283034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142010.22660236080693483No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC113660.18136486394842766No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC106230.16950896971002527No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG84180.13432424993118636No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA83450.13315940433306606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.12345767661173543No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC66250.10571372722667016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG230200.027.7664221
TACATGG236300.026.7917562
ACATGGG240100.025.3855273
CATGGGG166250.024.422524
TACCTGG47700.024.2389622
ATGGGGG104450.022.3155775
GAGTACT168000.020.31635912-13
GTACCTG59500.020.2361931
TGGGGGG94300.019.2856456
AGAGTAC273200.018.87742610-11
GTACTTT180250.018.7625114-15
GTATAGG17250.018.2680231
AGTACTT177850.017.72407212-13
ACCTGGG64550.017.5452333
GAGTACA177000.016.3952371
AGTACAT170600.015.9237782
ACTTTTT220050.015.6650216-17
GTATATA26700.015.5016251
CATGGGA86800.015.2133534
TACTTTT207450.014.97695214-15
Produced by FastQC (version 0.11.5)