FastQCFastQC Report
Mon 6 Jun 2016
ERR523169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920217
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349310.44103589585992403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288120.3637779116405523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168250.21243104829072232No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146760.18529795332627882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123200.050.8822
GTACCTG145900.043.3649021
ACCTGGG141850.043.0984343
CCTGGGG128050.033.0861244
GGTATCA297450.027.754571
GTATCAA417600.026.8882981
CATGGGG233950.025.3651244
TATCACG6700.025.2680932
TATAACG7850.025.1607972
GTACATG389200.024.9464191
TACATGG392100.024.3709012
CTGGGGG84700.024.3144195
ATGGGGG156650.024.2523695
TGGGGGG168550.023.6716926
ACATGGG398800.023.3602163
TCAACGC478800.023.1868934
CAACGCA483850.023.0114575
TATCAAC485100.022.897782
ATCAACG484450.022.8606973
AACGCAG495900.022.582936
Produced by FastQC (version 0.11.5)