FastQCFastQC Report
Mon 6 Jun 2016
ERR523169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920217
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308240.3891812560186167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274950.34714957936127255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162470.20513326844453883No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128670.16245767003606087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG126050.053.501672
GTACCTG145950.046.5517431
ACCTGGG145200.045.3733983
CCTGGGG125650.035.154854
GGTATCA281400.028.4051481
CTGGGGG83250.026.868115
GTATCAA393000.026.4645671
TGGGGGG147450.024.9536536
ATGGGGG144700.023.1869755
CATGGGG222950.023.0162774
GTACATG368050.022.8163851
TATCACG7250.022.6875882
TCAACGC457450.022.4146024
ATCAACG461800.022.213643
CAACGCA463600.022.1271115
TATCAAC467400.022.0801472
TACATGG375750.022.0126342
AACGCAG475050.021.7818686
TATAACG8500.021.5627422
ACATGGG380300.021.1665943
Produced by FastQC (version 0.11.5)