FastQCFastQC Report
Mon 6 Jun 2016
ERR523168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28663214
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1063520.37104003758964366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674380.2352771744299156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560360.19549796474324196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310780.10842468677797264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG1070050.028.5752981
TACATGG1085300.027.7106192
TACCTGG272600.027.2575442
ACATGGG1106500.026.3719843
CATGGGG704250.024.2264524
ACCTGGG337800.021.2723833
ATGGGGG372600.019.8399665
GTACCTG388900.019.8193261
GAGTACA814550.019.508251
GAGTACT663200.019.39673812-13
AGTACAT769150.019.1193142
GTACTTT694800.018.24477414-15
AGAGTAC1127750.018.19462410-11
CGCCGTA161550.018.00453894
CCGTATC117450.018.00353694
GTATAGG75050.017.6077371
CGTATCA90150.017.46133494
GCCGTAT141050.017.09002194
AGTACTT704100.017.05178812-13
TCGCCGT221050.016.47439694
Produced by FastQC (version 0.11.5)