FastQCFastQC Report
Mon 6 Jun 2016
ERR523168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28663214
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1061750.37042252135437426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT636130.22193254392197612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569310.19862043384248534No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT323080.11271590129425123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315710.11014466137677373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG276600.027.1218052
GTACATG1050300.027.0902351
TACATGG1072450.026.18342
ACATGGG1090400.025.0701373
CATGGGG683100.023.029734
ACCTGGG340850.021.3464583
GTACCTG399000.019.3454231
ATGGGGG354700.019.0821065
GAGTACT688400.018.99631112-13
GTACTTT698750.018.4322214-15
GAGTACA830900.018.3020421
AGTACAT778200.017.7700772
TAGATCG22750.017.5615375
AGAGTAC1167450.017.54600710-11
AGTACTT725550.016.77997212-13
GTATAGG77550.015.8913971
TATACAG144150.015.29266555
CATGGGT213000.015.2260384
GTATCAA1963800.015.2048531
CATGGGA382800.015.0780034
Produced by FastQC (version 0.11.5)