FastQCFastQC Report
Mon 6 Jun 2016
ERR523167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7562706
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT769411.0173739399627595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277190.36652224746010226No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT222600.29433908973851425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203510.2690968021234727No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC164110.21699904769536196No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC150370.1988309475470817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129800.17163168844590812No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG120690.15958573558194647No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA120090.15879236876324426No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA112990.14940419474193495No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC97770.12927912310752263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG60900.030.107072
GTACATG264350.027.798111
TACATGG270250.026.6162512
ACATGGG273700.025.6975883
GTACCTG74450.025.5124551
TATCACG6650.024.7438682
CATGGGG191050.024.5070024
ATGGGGG120900.023.213645
ACCTGGG77450.023.1887593
TATAACG7100.023.1755942
GAGTACT236450.020.32768412-13
AGAGTAC357700.019.14069410-11
TAACGCA8150.018.457394
ATAACGC8200.018.347283
TGGGGGG127000.018.3116976
AGTACTT248850.017.96481512-13
GTACTTT270300.017.66081814-15
GAGTACG11300.016.6424451
GAGTACA205550.016.1938951
TATACCG4451.3278623E-1015.8462495
Produced by FastQC (version 0.11.5)