FastQCFastQC Report
Mon 6 Jun 2016
ERR523167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7562706
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT784691.037578348279042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251910.33309505883211643No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT223820.29595226893654203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197210.2607664505270997No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC174580.23084329868171524No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC154830.20472830756610136No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA123590.16342034187234042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123380.16314266348579465No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG122830.16241541056865094No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA115810.15313301878983526No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC104160.137728479726701No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62950.033.896272
GTACCTG77700.028.0836181
GTACATG256050.027.9541281
TACATGG262800.026.5757332
TATAACG6900.026.564852
ACATGGG264200.025.6494643
ACCTGGG80300.025.39923
CATGGGG182600.025.0928344
ATGGGGG118550.024.061915
TATCACG7350.021.1017462
TGGGGGG117300.020.7926336
ATAACGC8500.019.90353
GAGTACT240300.019.85829512-13
AGAGTAC353850.018.8535410-11
CCTGGGG78400.018.4620764
TAACGCA8300.018.1183134
GGGTTAG24300.017.8048231
GTACTTT266350.017.77146114-15
GTGTAGC39600.017.4573461
AGTACTT253550.017.2815212-13
Produced by FastQC (version 0.11.5)