FastQCFastQC Report
Mon 6 Jun 2016
ERR523166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5813783
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218930.3765706425575224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169660.29182375743986316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97920.16842733896328776No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.1096016139577277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG91550.053.163892
ACCTGGG101250.046.3057483
TATAACG6900.040.2103352
GTACCTG123550.040.1928181
GGTATCA154450.035.6834641
TAACGCA8050.033.8815424
CCTGGGG92100.032.9329874
ATAACGC8450.032.8344733
TATCACG5050.032.5920332
GTATCAA231450.030.212111
TCAACGC269600.025.7627144
CAACGCA274100.025.3905725
ATCAACG276250.025.1087173
AACGCAG283600.024.7130726
TATCAAC281000.024.7010152
GTACATG222250.024.4381051
TACATGG225900.023.5440482
CTGGGGG56250.023.4075155
ACATGGG222050.022.8298343
CATGGGG133100.022.5410234
Produced by FastQC (version 0.11.5)