FastQCFastQC Report
Mon 6 Jun 2016
ERR523166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5813783
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202400.348138208804835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172040.29591747748410974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97680.1680145268579856No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT87080.14978199220713947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG96100.052.139572
ACCTGGG101900.047.7872163
GTACCTG128700.039.614091
TATAACG7250.036.9547652
TAACGCA7650.036.8651474
CCTGGGG89250.034.8638954
GGTATCA157400.033.4068871
ATAACGC8600.032.246293
GTATCAA232650.030.4453431
TATCACG4700.028.0023042
CTGGGGG52200.025.8431245
TCAACGC271000.025.8256664
CAACGCA275600.025.4121075
ATCAACG275300.025.3369223
AACGCAG284900.024.7454346
TATCAAC282800.024.6487752
GTACATG209450.023.757691
CATGGGG122500.022.7149494
TACATGG209350.022.654362
ATGGGGG77150.022.5423625
Produced by FastQC (version 0.11.5)