FastQCFastQC Report
Mon 6 Jun 2016
ERR523165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7629663
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284400.3727556511997974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224800.29463948800884127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127330.16688810501853096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG85450.038.635922
GTACCTG105200.032.1869741
ACCTGGG104100.030.5844923
GTATCAA323200.029.465721
GGTATCA223750.029.089511
TATAACG6900.027.94482
GTACATG272500.025.6285061
TCAACGC376400.025.0135294
CAACGCA379400.024.7397445
TATCAAC388650.024.4311492
ATCAACG385800.024.3189073
TACATGG281000.024.2341882
AACGCAG393000.024.065436
ACATGGG281050.023.476723
CATGGGG189100.022.4323884
ACGCAGA435250.021.6520797
CGCAGAG447300.021.0364348
GAGTACT236000.020.54254312-13
CCTGGGG107850.020.0148834
ATGGGGG106050.019.466415
Produced by FastQC (version 0.11.5)