FastQCFastQC Report
Mon 6 Jun 2016
ERR523165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7629663
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271000.35519262122062273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233900.30656662030813153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128920.16897207648620916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG89800.037.3199462
ACCTGGG103700.031.2742393
GTACCTG111400.030.9473061
GGTATCA241200.029.9319571
GTATCAA342600.028.129281
TCAACGC397500.023.9680714
CAACGCA403700.023.623725
ATCAACG407200.023.3971213
GTACATG276900.023.355211
TATCAAC413700.023.1778132
CCTGGGG107200.023.018714
AACGCAG417250.023.01236
TATAACG6850.022.6438982
TACATGG283600.022.3911782
TAACGCA8850.021.7749444
ACATGGG282600.021.704753
GAGTACT249300.020.68083412-13
ACGCAGA466950.020.6239077
CGCAGAG478800.020.1426648
CATGGGG187400.019.738844
Produced by FastQC (version 0.11.5)