FastQCFastQC Report
Mon 6 Jun 2016
ERR523164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080281
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70820.22991408900681462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.18816465121201606No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34910.11333381597328296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32250.10469824019302135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG30550.026.6303372
CGTATCA11850.026.1727894
GTACATG111350.026.1417791
CATGGGG68800.025.6992244
TACATGG112700.025.1614232
ACATGGG114300.024.1505263
GTATCAA131750.023.700211
ATGGGGG43850.022.4121345
CCGTATC16100.021.59882494
GGTATCA99400.021.5258241
ACCTGGG36500.021.129333
TCAACGC148950.020.7733254
CAACGCA149300.020.6294655
ATCAACG151850.020.3462943
TATCAAC152050.020.3198592
AACGCAG153300.020.1743566
TATAACG2201.4818652E-619.2380752
GTACCTG45000.018.8102571
GAGTACT73600.018.3898312-13
CGCCGTA23050.018.14442894
Produced by FastQC (version 0.11.5)