FastQCFastQC Report
Mon 6 Jun 2016
ERR523164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080281
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT146200.4746320222083634No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT79940.259521777396283RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.21608418193015508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60270.1956639670211906No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT42110.13670830680707377RNA PCR Primer, Index 27 (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34800.11297670569665559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG28000.026.1885032
GTACATG109350.025.2093431
GGTATCA98050.024.7562471
TACATGG110750.024.319522
ACATGGG112000.023.0809423
GTATCAA127750.023.0513381
CATGGGG68750.021.3983614
TCAACGC142450.020.3908314
CAACGCA144250.020.006385
GTATAGG7600.019.8070181
ATCAACG146600.019.781543
GAGTACT72150.019.73790612-13
TATCAAC148850.019.7367362
AACGCAG148450.019.4710666
ATGGGGG40450.019.405015
ACCTGGG37100.019.3832423
GTACTTT74700.019.12595414-15
GTGTAGC17400.018.38411
GTACCTG43350.017.9051321
ACGCAGA164750.017.6593277
Produced by FastQC (version 0.11.5)