FastQCFastQC Report
Mon 6 Jun 2016
ERR523163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10714663
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265610.24789393749481436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206020.1922785625642169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128050.11950912501867768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200850.035.4943581
GTATCAA317250.034.3522721
TCAACGC370450.028.9236154
CAACGCA374550.028.593525
ATCAACG379450.028.315213
GTACATG340550.027.9118771
AACGCAG385800.027.802086
TATCAAC391250.027.6095162
TACATGG349550.026.3443982
ACATGGG355300.025.2659853
ACGCAGA438550.024.4366577
CGCAGAG453100.023.6920918
CATGGGG240650.023.0822934
GCAGAGT505350.020.9256159
GAGTACT259050.020.56700912-13
AGAGTAC400050.020.54407310-11
ATGGGGG137200.018.4615355
GTACTTT285750.018.37401214-15
AGTACTT282700.018.01545112-13
GAGTACA261950.017.8381331
Produced by FastQC (version 0.11.5)