FastQCFastQC Report
Mon 6 Jun 2016
ERR523163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10714663
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234400.21876562986628698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196210.18312288496614407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121630.11351733600954131No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200700.035.2553861
GTATCAA309550.032.5698971
TCAACGC361250.027.330684
CAACGCA368300.026.8457955
ATCAACG369400.026.7157173
AACGCAG380050.025.9908316
GTACATG343050.025.7962381
TATCAAC386200.025.7561592
TACATGG351100.024.6205482
ACATGGG355750.023.7249813
ACGCAGA435700.022.595437
CGCAGAG452750.021.7494898
CATGGGG241400.021.2577674
AGAGTAC401400.019.39154810-11
GCAGAGT506300.019.2911939
GAGTACT260450.019.23815512-13
GTACTTT278750.017.76256414-15
AGTACTT280250.017.54351612-13
GAGTACA263150.017.2881361
CAGAGTA491050.016.8994110-11
Produced by FastQC (version 0.11.5)