FastQCFastQC Report
Mon 6 Jun 2016
ERR523162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4684449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183840.3924474361872656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136510.29141100692952365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92100.19660796819433832No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70790.15111702571636493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59500.052.5308152
TATAACG6250.043.617172
GGTATCA87250.043.0321851
ACCTGGG72850.042.12523
GTACCTG78850.040.0980721
GTATCAA158250.039.988441
TAACGCA7600.035.2441524
TCAACGC190400.032.652674
GTACATG175400.032.4304241
ATCAACG193500.032.1076743
CAACGCA194900.031.8736295
ATAACGC8300.031.707963
CCTGGGG62300.031.529284
TATCAAC198950.031.3735472
AACGCAG203800.030.7995556
TACATGG182050.030.5940632
ACATGGG183250.029.3130233
CATGGGG123550.027.8415974
ACGCAGA225000.027.7722647
CGCAGAG232750.026.8264718
Produced by FastQC (version 0.11.5)