FastQCFastQC Report
Mon 6 Jun 2016
ERR523162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4684449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165760.35385164829417504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130670.2789442258844103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84510.18040542228125442No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62730.1339111600958832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58950.054.739732
TATAACG5850.053.070162
ACCTGGG71700.044.1108553
GTACCTG78300.042.184581
GGTATCA90450.042.1361471
TAACGCA8000.039.9447024
TATCACG5250.038.5275572
GTATCAA156700.038.4043051
ATAACGC8350.037.1455733
CCTGGGG61050.032.252894
TCAACGC188300.031.3208644
CAACGCA191500.030.8465655
ATCAACG191450.030.8061873
TATCAAC196000.030.3356552
GTACATG168500.030.2138251
AACGCAG200850.029.7612346
TACATGG176850.028.194422
ACATGGG175200.027.520913
CATGGGG114700.027.2867034
ACGCAGA224450.026.610747
Produced by FastQC (version 0.11.5)